CD4+ CD25+ T cells in skin lesions of patients with cutaneous leishmaniasis exhibit phenotypic and functional characteristics of natural regulatory T cells

被引:145
作者
Campanelli, AP
Roselino, AM
Cavassani, KA
Pereira, MSF
Mortara, RA
Brodskyn, CI
Gonçalves, HS
Belkaid, Y
Barral-Netto, M
Barral, A
Silva, JS
机构
[1] Univ Sao Paulo, Sch Med Ribeirao Preto, Dept Biochem & Immunol, BR-14049900 Ribeirao Preto, SP, Brazil
[2] Univ Sao Paulo, Sch Med Ribeirao Preto, Div Dermatol, BR-14049900 Ribeirao Preto, SP, Brazil
[3] Univ Fed Sao Paulo, Dept Microbiol Immunol & Parasitol, Sao Paulo, Brazil
[4] Goncalo Moniz Res Inst, Fortaleza, Ceara, Brazil
[5] Inst Invest Immunol, Fortaleza, Ceara, Brazil
[6] Ctr Dermatol Dona Libana, Fortaleza, Ceara, Brazil
[7] NIH, Mucosal Immunol Unit, Parasit Dis Lab, Bethesda, MD 20892 USA
基金
巴西圣保罗研究基金会;
关键词
D O I
10.1086/502980
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Endogenous regulatory T ( T-reg) cells are involved in the control of infections, including Leishmania infection in mice. Leishmania viannia braziliensis is the main etiologic agent of cutaneous leishmaniasis ( CL) in Brazil, and it is also responsible for the more severe mucocutaneous form. Here, we investigated the possible involvement of T-reg cells in the control of the immune response in human skin lesions caused by L. viannia braziliensis infection. We show that functional T-reg cells can be found in skin lesions of patients with CL. These cells express phenotypic markers of T-reg cells-such as CD25, cytotoxic T lymphocyte-associated antigen 4, Foxp3, and glucocorticoid-induced tumor necrosis factor receptor-and are able to produce large amounts of interleukin-10 and transforming growth factor-beta. Furthermore, CD4(+) CD25(+) T cells derived from the skin lesions of 4 of 6 patients with CL significantly suppressed in vitro the phytohemagglutinin-induced proliferative T cell responses of allogeneic peripheral-blood mononuclear cells ( PBMCs) from healthy control subjects at a ratio of 1 T-reg cell to 10 allogeneic PBMCs. These findings suggest that functional Treg cells accumulate at sites of Leishmania infection in humans and possibly contribute to the local control of effector T cell functions.
引用
收藏
页码:1313 / 1322
页数:10
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