Isolation of high-quality RNA from gymnosperm and angiosperm trees

被引：134

作者：

Kolosova, N ^{[1
]}

Miller, B ^{[1
]}

Ralph, S ^{[1
]}

Ellis, BE ^{[1
]}

Douglas, C ^{[1
]}

Ritland, K ^{[1
]}

Bohlmann, J ^{[1
]}

机构：

[1] Univ British Columbia, Biomed Res Ctr, Vancouver, BC V6T 1Z3, Canada

来源：

BIOTECHNIQUES | 2004年 / 36卷 / 05期

关键词：

D O I：

10.2144/04365ST06

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An improved protocol was developed for efficient and reliable extraction of high-quality total RNA and mRNA from various tissues ofspruce (Picea spp.) and poplar (Populus spp.) trees, as well as other plant species. This method was specifically optimized for tissues with high content of polysaccharides, oleoresin terpenoids, and phenolic secondary metabolites, which often co-precipitate with RNA and inhibit subsequent reverse transcription. The improved protocol yielded up to 600mug of total RNA per gram of tissue suitable for standard expressed sequence tags (ESTs), full-length cDNA library construction, and for microarray applications.

引用

页码：821 / 824

页数：4

共 8 条

[1] A simple procedure for the isolation of high quality RNA from ripening banana fruit [J].

Asif, MH ;

Dhawan, P ;

Nath, P .

PLANT MOLECULAR BIOLOGY REPORTER, 2000, 18 (02) :109-115

[2]

Carninci P, 1999, METHOD ENZYMOL, V303, P19

[3]

Chang S. J., 1993, Plant Molecular Biology Reporter, V11, P113, DOI 10.1007/BF02670468

[4] A simple and efficient protocol for isolation of functional RNA from plant tissues rich in secondary metabolites [J].

Kiefer, E ;

Heller, W ;

Ernst, D .

PLANT MOLECULAR BIOLOGY REPORTER, 2000, 18 (01) :33-39

[5]

Lewinsohn E., 1994, Plant Molecular Biology Reporter, V12, P20, DOI 10.1007/BF02668660

[6] Isolation of high-quality RNA from white spruce tissue using a three-stage purification method and subsequent cloning of a transcript from the PR-10 gene family [J].

Mattheus, N ;

Ekramoddoullah, AKM ;

Lee, SP .

PHYTOCHEMICAL ANALYSIS, 2003, 14 (04) :209-215

[7] Isolation and purification of functional total RNA from woody branches and needles of Sitka and white spruce [J].

Wang, SX ;

Hunter, W ;

Plant, A .

BIOTECHNIQUES, 2000, 28 (02) :292-+

[8] A quick and easy method for isolating good-quality RNA from cotton (Gossypium hirsutum L.) tissues [J].

Wu, YR ;

Llewellyn, DJ ;

Dennis, ES .

PLANT MOLECULAR BIOLOGY REPORTER, 2002, 20 (03) :213-218

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