Evaluation of antioxidative capability of the tomato (Solanum lycopersicum) skin constituents by capillary electrophoresis and high-performance liquid chromatography

被引:41
作者
Helmja, Kati [1 ]
Vaher, Merike [1 ]
Pussa, Toenu [2 ]
Raudsepp, Piret [2 ]
Kaljurand, Mihkel [1 ]
机构
[1] Tallinn Univ Technol, Fac Sci, Dept Chem, EE-12618 Tallinn, Estonia
[2] Estonian Univ Life Sci, Dept Food Sci & Hyg, Tartu, Estonia
关键词
Antioxidative capability; Capillary electrophoresis; Flavonoids; Free radical scavenging;
D O I
10.1002/elps.200800012
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
In the current study, phenolic compounds of the tomato (Solanum lycopersicum) skin extract were separated and their composition was determined by capillary electrophoresis and tandem high-performance liquid chromatography diode array detection-mass spectrometry (HPLC-DAD-MS/MS). Both the total phenolic and flavonoid contents of the extract were determined. The antioxidative capability of the extract was measured using a stable free radical 2,2-diphenyl-1-picrylhydrazyl assay. The monitoring of the radical scavenging capability of specific phenolic compounds was carried out both by capillary electrophoresis and HPLC-MS/MS.
引用
收藏
页码:3980 / 3988
页数:9
相关论文
共 54 条
[1]   Total phenol, flavonoid, proanthocyanidin and vitamin C levels and antioxidant activities of Mauritian vegetables [J].
Bahorun, T ;
Luximon-Ramma, A ;
Crozier, A ;
Aruoma, OI .
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE, 2004, 84 (12) :1553-1561
[2]   Improving the in vivo predictability of an on-line HPLC stable free radical decoloration assay for antioxidant activity in methanol-buffer medium [J].
Bartasiute, Aiste ;
Westerink, Ben H. C. ;
Verpoorte, Elisabeth ;
Niederlaender, Harm A. G. .
FREE RADICAL BIOLOGY AND MEDICINE, 2007, 42 (03) :413-423
[3]   Phenol content related to antioxidant and antimicrobial activities of Passiflora spp. extracts [J].
Bendini, Alessandra ;
Cerretani, Lorenzo ;
Pizzolante, Luca ;
Toschi, Tullia Gallina ;
Guzzo, Flavia ;
Ceoldo, Stefania ;
Marconi, Andrea Maria ;
Andreetta, Filippo ;
Levi, Marisa .
EUROPEAN FOOD RESEARCH AND TECHNOLOGY, 2006, 223 (01) :102-109
[4]   LC coupled to ion-trap MS for the rapid screening and detection of polyphenol antioxidants from Helichrysum stoechas [J].
Carini, M ;
Aldini, G ;
Furlanetto, S ;
Stefani, R ;
Facino, RM .
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 2001, 24 (03) :517-526
[5]   Comparisons on the antioxidant properties of fresh, freeze-dried and hot-air-dried tomatoes [J].
Chang, Ching-Hui ;
Lin, Hsing-Yu ;
Chang, Chi-Yue ;
Liu, Yung-Chuan .
JOURNAL OF FOOD ENGINEERING, 2006, 77 (03) :478-485
[6]   Hydroxyl radical in living systems and its separation methods [J].
Cheng, FC ;
Jen, JF ;
Tsai, TH .
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2002, 781 (1-2) :481-496
[7]   Contents of polyphenols in fruit and vegetables [J].
Cieslik, E ;
Greda, A ;
Adamus, W .
FOOD CHEMISTRY, 2006, 94 (01) :135-142
[8]   Recent advances in the application of capillary electromigration methods for food analysis [J].
Cifuentes, A .
ELECTROPHORESIS, 2006, 27 (01) :283-303
[9]   The chlorogenic acids of Hemerocallis [J].
Clifford, MN ;
Wu, WG ;
Kuhnert, N .
FOOD CHEMISTRY, 2006, 95 (04) :574-578
[10]   Analytical separation and detection methods for flavonoids [J].
de Rijke, E ;
Out, P ;
Niessen, WMA ;
Ariese, F ;
Gooijer, C ;
Brinkman, UAT .
JOURNAL OF CHROMATOGRAPHY A, 2006, 1112 (1-2) :31-63