A novel subpopulation of monocyte-like cells in the human lung after lipopolysaccharide inhalation

被引：26

作者：

Brittan, Mairi ^{[1
]}

Barr, Laura ^{[1
]}

Morris, Andrew Conway ^{[1
]}

Duffin, Rodger ^{[1
]}

Rossi, Fiona ^{[1
]}

Johnston, Shonna ^{[1
]}

Monro, Graham ^{[1
]}

Anderson, Niall ^{[2
]}

Rossi, Adriano G. ^{[1
]}

McAuley, Danny F. ^{[3
]}

Haslett, Christopher ^{[1
]}

Hirani, Nik ^{[1
]}

Dhaliwal, Kev ^{[1
]}

Simpson, A. John ^{[1
,4
]}

机构：

[1] Univ Edinburgh, MRC Ctr Inflammat Res, Queens Med Res Inst, Edinburgh EH16 4TJ, Midlothian, Scotland

[2] Univ Edinburgh, Ctr Populat Hlth Sci, Sch Med, Edinburgh EH16 4TJ, Midlothian, Scotland

[3] Queens Univ Belfast, Ctr Infect & Immun, Belfast, Antrim, North Ireland

[4] Newcastle Univ, Inst Cellular Med, Sch Med, Newcastle Upon Tyne NE1 7RU, Tyne & Wear, England

来源：

EUROPEAN RESPIRATORY JOURNAL | 2012年 / 40卷 / 01期

基金：

英国医学研究理事会;

关键词：

Acute lung inflammation; macrophages; monocytes; multiparameter flow cytometry; regulatory T-cells; ALVEOLAR MACROPHAGES; INHALED ENDOTOXIN; LPS INHALATION; BLOOD; INFLAMMATION; POPULATION; EXPRESSION; PHENOTYPE;

D O I：

10.1183/09031936.00113811

中图分类号：

R56 [呼吸系及胸部疾病];

学科分类号：

100201 [内科学];

摘要：

The co-ordinated recruitment of monocyte subpopulations, neutrophils and regulatory T-cells (Tregs) during the early stages of human acute lung inflammation remains poorly understood. We therefore performed a detailed characterisation of these lineages in the blood and lungs in a model of human acute lung inflammation. Healthy volunteers inhaled lipopolysaccharide (LPS) or saline (n=6 for each group). Blood was collected at 0, 2, 4, 6 and 8 h and bronchoscopy with bronchoalveolar lavage (BAL) performed at 8 h. Multiparameter flow cytometry was used to characterise monocyte subpopulations, neutrophils and Tregs in the blood and lung. Inhalation of LPS was associated with significant blood and BAL fluid neutrophilia. Blood populations of monocyte subpopulations and Tregs were unaltered by LPS. In contrast, LPS induced an accumulation of a pulmonary monocyte-like cell (PMLC) population, which was further subdivided into "inducible'' CD14(++)CD16(-) and "resident'' CD14(++)CD16(+) subsets. Inducible PMLCs were significantly increased following LPS inhalation (p=0.0046), whereas resident PMLCs were unchanged. In addition, we noted a significant decrease in Tregs in BAL fluid with LPS inhalation (p=0.027). The early stages of LPS-induced inflammation in humans is characterised by pulmonary accumulation of a novel inducible monocyte-like subpopulation, accompanied by significant changes in both neutrophil and Treg numbers.

引用

页码：206 / 214

页数：9

共 33 条

[1]

Acute LPS inhalation in healthy volunteers induces dendritic cell maturation in vivo [J].