Dynamics of individual flexible polymers in a shear flow

Polymer dynamics are of central importance in materials science, mechanical engineering, biology and medicine(1,2). The dynamics of macromolecular solutions and melts in shear flow are typically studied using bulk experimental methods such as light and neutron scattering and birefringence(3,4). But the effect of shear on the conformation and dynamics of individual polymers is still not well understood(5-7). Here we describe observations of the real-time dynamics of individual, flexible polymers (fluorescently labelled DNA molecules(8-15)) under a shear flow. The sheared polymers exhibit many types of extended conformation with an overall orientation ranging from parallel to perpendicular with respect to the flow direction. For shear rates much smaller than the inverse of the relaxation time of the molecule, the relative populations of these two main types of conformation are controlled by the rate of the shear flow. These results question the adequacy of assumptions made in standard models of polymer dynamics(5,6).