Assessment of biochemical methods to detect enzymatic depolymerization of polysaccharides

被引：25

作者：

Rigouin, C. ^{[1
,2
]}

Ladrat, C. Delbarre ^{[1
]}

Sinquin, C. ^{[1
]}

Colliec-Jouault, S. ^{[1
]}

Dion, M. ^{[2
]}

机构：

[1] IFREMER, Lab Biotechnol & Mol Marines, F-44311 Nantes 3, France

[2] Univ Nantes, Fac Sci & Tech, CNRS, UMR 6204, F-44322 Nantes 3, France

来源：

CARBOHYDRATE POLYMERS | 2009年 / 76卷 / 02期

关键词：

Carbohydrate; Depolymerization; Enzyme; Detection; BACTERIAL EXOPOLYSACCHARIDE; MICROORGANISMS; LYASE; CHROMATOGRAPHY; ASSAY;

D O I：

10.1016/j.carbpol.2008.10.022

中图分类号：

O69 [应用化学];

学科分类号：

070301 [无机化学];

摘要：

Biochemical methods for detection of depolymerisation were compared. Currently used methods Such as reducing sugars assays, double bond monitoring or molecular weight determination were tested to follow the kinetic of depolymerization with different enzyme/polysaccharide combinations. The range of concentrations of different enzymes allowed us to identify the most sensitive and appropriate method to detect polysaccharide degradation. Reducing sugars assays are quantitative, sensitive and usable with all kind of polysaccharide but some Compounds may interfere With them. When the polysaccharide is charged, agarose gel electrophoresis, although being a qualitative assay is as sensitive as high performance size exclusion chromatography analysis, easy to handle, high-throughput and this is preferred. (C) 2008 Elsevier Ltd. All rights reserved.

引用

页码：279 / 284

页数：6

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