The incorporation of a photoisomerizable amino acid into proteins in E-coli

被引：141

作者：

Bose, M

Groff, D

Xie, JM

Brustad, E

Schultz, PG

机构：

[1] Scripps Res Inst, Dept Chem, La Jolla, CA 92037 USA

[2] Scripps Res Inst, Skaggs Inst Chem Biol, La Jolla, CA 92037 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2006年 / 128卷 / 02期

关键词：

D O I：

10.1021/ja055467u

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

An orthogonal aminoacyl tRNA synthetase/tRNA pair has been evolved that allows the incorporation of the photoisomerizable amino acid phenylalanine-4′-azobenzene (AzoPhe) into proteins in E. coli in response to the amber nonsense codon. Further, we show that AzoPhe can be used to photoregulate the binding affinity of catabolite activator protein to its promoter. The ability to selectively incorporate AzoPhe into proteins at defined sites should make it possible to regulate a variety of biological processes with light, including enzyme, receptor, and ion channel activity. Copyright © 2006 American Chemical Society.

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页码：388 / 389

页数：2

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