High-throughput preparative process utilizing three complementary chromatographic purification technologies

被引：42

作者：

Ventura, M

Farrell, W

Aurigemma, C

Tivel, K

Greig, M

Wheatley, J

Yanovsky, A

Milgram, KE

Dalesandro, D

DeGuzman, R

Tran, P

Nguyen, L

Chung, L

Gron, O

Koch, CA

机构：

[1] Pfizer Global R&D La Jolla, San Diego, CA 92121 USA

[2] Koch Associates, La Jolla, CA 92037 USA

来源：

JOURNAL OF CHROMATOGRAPHY A | 2004年 / 1036卷 / 01期

关键词：

preparative chromatography; supercritical fluid chromatography; parallel synthesis; instrumentation;

D O I：

10.1016/j.chroma.2004.02.071

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A high-throughput process was developed in which wells in plates generated from parallel synthesis are automatically channeled to an appropriate purification technique using analytical data as a guide. Samples are directed to either of three fundamentally different preparative techniques: HPLC with UV-triggered fraction collection, supercritical fluid chromatography (SFC) with UV-triggered fraction collection, or HPLC with MS-triggered fraction collection. Automated analysis of the analytical data identifies the product compound mass and creates work lists based on chromatographic properties exhibited in the data so that each preparative instrument cherry picks the appropriate list of samples to purify when a preparative-scale plate is loaded. (C) 2004 Elsevier B.V. All rights reserved.

引用

收藏

页码：7 / 13

页数：7

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