Drug screening study using glioma stem-like cells

被引：47

作者：

Feng, Xing ^{[1
]}

Zhou, Qin ^{[1
]}

Liu, Chong ^{[1
]}

Tao, Mei-Ling ^{[1
]}

机构：

[1] Hunan Normal Univ, Coll Med, Changsha 410006, Hunan, Peoples R China

来源：

MOLECULAR MEDICINE REPORTS | 2012年 / 6卷 / 05期

关键词：

glioma stem-like cells; glioma cells; MTT; TUMOR-INITIATING CELLS; CANCER-CELLS; MALIGNANT GLIOMA; TEMOZOLOMIDE; GROWTH; IDENTIFICATION; GLIOBLASTOMA; REPAIR;

D O I：

10.3892/mmr.2012.1040

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 [肿瘤学];

摘要：

Glioma is one of the most common malignant tumors affecting the central nervous system. We screened active drugs from 12 classes to provide experimental data for the study of human glioma. Serum-free suspension clone formation was used to induce the formation of glioma stem-like cells (GSLCs) from U251 cells. We screened active drugs from 12 classes and observed their effects on the number of tumor balls. The results revealed that serum-free suspension clone formation successfully induced the formation of GSLCs. Six active drugs [curcumin, chrysin, apigenin, luteolin, casticin and 8-bromo-7-methoxychrysin (BrMChR)] were identified by MTT assay. The drugs that affected the number of tumor balls were curcumin, luteolin, casticin and BrMChR (all P<0.05). The GSLCs were obtained by scrum-free clone suspension formation and exhibited the features of cancer stem cells.

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页码：1117 / 1120

页数：4

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