A cis-regulatory sequence within the yellow locus of Drosophila melanogaster required for normal male mating success

被引:56
作者
Drapeau, MD
Cyran, SA
Viering, MM
Geyer, PK
Long, AD
机构
[1] NYU, Dept Biol, Ctr Dev Genet, New York, NY 10003 USA
[2] Univ Calif Irvine, Dept Ecol & Evolutionary Biol, Irvine, CA 92697 USA
[3] Univ Iowa, Dept Biochem, Iowa City, IA 52242 USA
关键词
D O I
10.1534/genetics.105.045666
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Drosophila, melanogaster males perform a courtship ritual consisting of a series of dependent fixed-action patterns. The yellow (y) gene is required for normal male courtship behavior and subsequent mating success. To better characterize the requirement for y in the manifestation of innate male sexual behavior; we measured the male mating success (MMS) of 12 hypomorphic y mutants and matched-outbred-background controls using a y(+) rescue element on a freely segregating minichromosome. We found that 4 hypomorphs significantly reduced DIMS to varying degrees. Reduced MMS was largely independent of adult pigmentation patterns. These mutations defined a 300-bp regulatory region upstream of the transcription start, the mating-success regulatory sequence (MRS), whose function is required for normal MMS. Visualization of gene action via GFP and a Yellow antibody suggests that the MRS directs y transcription in a small number of cells in the third instar CNS, the developmental stage previously implicated in the role of y with regard to male courtship behavior. The presence of Yellow protein in these cells positively correlates With MMS in a subset of mutants. The MRS contains a regulatory sequence controlling larval pigmentation and a 35-bp sequence that is highly conserved within the genus Drosophila and is predicted to bind known transcription factors.
引用
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页码:1009 / 1030
页数:22
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