Identification and characterization of microsatellite markers in the Chagas disease vector Triatoma dimidiata

被引：21

作者：

Anderson, Jennifer M. ^{[1
,2
]}

Lai, James E. ^{[3
]}

Dotson, Ellen M. ^{[1
]}

Cordon-Rosales, Celia ^{[4
,5
]}

Ponce, Carlos ^{[6
]}

Norris, Douglas E. ^{[2
]}

Ben Beard, C. ^{[1
]}

机构：

[1] Ctr Dis Control & Prevent, Div Parasit Dis, Natl Ctr Infect Dis, Entomol Branch, Atlanta, GA 30341 USA

[2] Johns Hopkins Bloomberg Sch Publ Hlth, Dept Microbiol & Immunol, Baltimore, MD 21205 USA

[3] Ctr Dis Control & Prevent, Publ Hlth Prevent Serv, Epidemiol Program Off, Atlanta, GA 30333 USA

[4] Univ Valle Guatemala, Inst Invest, Guatemala City, Guatemala

[5] CDC, Med Entomol Res & Training Unit, Guatemala City, Guatemala

[6] Secretaria Salud, Lab Cent Referencia Chagas & Leishmanioses, Tegucigalpa, Honduras

来源：

INFECTION GENETICS AND EVOLUTION | 2002年 / 1卷 / 03期

关键词：

Chagas disease; Microsatellites; Triatoma;

D O I：

10.1016/S1567-1348(02)00033-3

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Triatoma dimidiata, one of the major vectors of Chagas disease in Central America, is found in both domestic and peri-domestic habitats. Questions concerning population boundaries, infestation rates, insecticide resistance, and geographic dispersal of triatomine bugs persist and may be resolved using genetic markers such as microsatellites. Microsatellites are short tandem repeats found dispersed throughout a genome and can be useful for genotypic identification. We developed a plasmid library from the genomic DNA isolated from a single T. dimidiata adult collected in Guatamala. Ten thousand clones were screened using a probe consisting of nine microsatellite oligonucleotides. Eight loci appear polymorphic among populations found in Guatemala, Honduras, and Mexico, and thus are potentially useful for population genetic applications. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：243 / 248

页数：6

共 24 条

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