Hydrocyanines: A Class of Fluorescent Sensors That Can Image Reactive Oxygen Species in Cell Culture, Tissue, and In Vivo

被引：281

作者：

Kundu, Kousik ^{[1
,2
]}

Knight, Sarah F. ^{[3
]}

Willett, Nick ^{[3
]}

Lee, Scingmun ^{[1
,2
]}

Taylor, W. Robert ^{[1
,2
,3
]}

Murthy, Niren ^{[1
,2
]}

机构：

[1] Georgia Inst Technol, Wallace H Coulter Dept Biomed Engn, Atlanta, GA 30332 USA

[2] Georgia Inst Technol, Parker H Petit Inst Bioengn & Biosci, Atlanta, GA 30332 USA

[3] Emory Univ, Sch Med, Dept Med, Div Cardiol, Atlanta, GA 30322 USA

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2009年 / 48卷 / 02期

关键词：

cyanines; fluorescence; hydrocyanines; reactive oxygen species; superoxide; HYDROGEN-PEROXIDE; LIVING CELLS; SELECTIVE DETECTION; SUPEROXIDE; PROBE; MACROPHAGES; DESIGN; HYDROETHIDINE; PRODUCT;

D O I：

10.1002/anie.200804851

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Accurate and tunable: The title compounds can detect reactive oxygen species (ROS) in cell culture, tissue explants, and for the first time in vivo. The hydrocyanines are synthesized by reduction of the cyanine dyes with NaBH4. They can accurately detect nanomolar levels of ROS, have excellent stability against autoxidation, and have tunable emission wavelengths in the range 560-830 nm. (Figure Presented). © 2009 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

页码：299 / 303

页数：5

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