Comparative genomics in Chlamydomonas and Plasmodium identifies an ancient nuclear envelope protein family essential for sexual reproduction in protists, fungi, plants, and vertebrates

被引：73

作者：

Ning, Jue ^{[1
]}

Otto, Thomas D. ^{[2
]}

Pfander, Claudia ^{[2
]}

Schwach, Frank ^{[2
]}

Brochet, Mathieu ^{[2
]}

Bushell, Ellen ^{[2
]}

Goulding, David ^{[2
]}

Sanders, Mandy ^{[2
]}

Lefebvre, Paul A. ^{[3
]}

Pei, Jimin ^{[4
,5
]}

Grishin, Nick V. ^{[4
,5
]}

Vanderlaan, Gary ^{[1
]}

Billker, Oliver ^{[2
]}

Snell, William J. ^{[1
]}

机构：

[1] Univ Texas SW Med Sch, Dept Cell Biol, Dallas, TX 75390 USA

[2] Wellcome Trust Sanger Inst, Cambridge CB10 1SA, England

[3] Univ Minnesota, Dept Plant Biol, St Paul, MN 55108 USA

[4] Univ Texas SW Med Ctr Dallas, Howard Hughes Med Inst, Dallas, TX 75390 USA

[5] Univ Texas SW Med Ctr Dallas, Dept Biochem, Dallas, TX 75390 USA

来源：

GENES & DEVELOPMENT | 2013年 / 27卷 / 10期

基金：

英国医学研究理事会; 英国惠康基金; 美国国家卫生研究院;

关键词：

RNA sequencing; fertilization; Plasmodium; Chlamydomonas; KAR5/GEX1/Brambleberry; nuclear envelope fusion; RODENT MALARIA PARASITE; MATING-TYPE; HSP70A PROMOTER; BLOOD STAGES; REINHARDTII; EXPRESSION; TRANSMISSION; FUSION; GAMETOGENESIS; FERTILIZATION;

D O I：

10.1101/gad.212746.112

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Fertilization is a crucial yet poorly characterized event in eukaryotes. Our previous discovery that the broadly conserved protein HAP2 (GCS1) functioned in gamete membrane fusion in the unicellular green alga Chlamydomonas and the malaria pathogen Plasmodium led us to exploit the rare biological phenomenon of isogamy in Chlamydomonas in a comparative transcriptomics strategy to uncover additional conserved sexual reproduction genes. All previously identified Chlamydomonas fertilization-essential genes fell into related clusters based on their expression patterns. Out of several conserved genes in a minus gamete cluster, we focused on Cre06.g280600, an ortholog of the fertilization-related Arabidopsis GEX1. Gene disruption, cell biological, and immunolocalization studies show that CrGEX1 functions in nuclear fusion in Chlamydomonas. Moreover, CrGEX1 and its Plasmodium ortholog, PBANKA_113980, are essential for production of viable meiotic progeny in both organisms and thus for mosquito transmission of malaria. Remarkably, we discovered that the genes are members of a large, previously unrecognized family whose first-characterized member, KAR5, is essential for nuclear fusion during yeast sexual reproduction. Our comparative transcriptomics approach provides a new resource for studying sexual development and demonstrates that exploiting the data can lead to the discovery of novel biology that is conserved across distant taxa.

引用

页码：1198 / 1215

页数：18

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