Micellar electrokinetic capillary chromatography for the determination of fluoxetine and its metabolite norfluoxetine in biological fluids

被引：23

作者：

Nevado, JJB ^{[1
]}

Salcedo, AMC ^{[1
]}

Llerena, MJV ^{[1
]}

机构：

[1] Univ Castilla La Mancha, Dept Analyt Chem & Food Technol, Ciudad Real 13071, Spain

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2002年 / 769卷 / 02期

关键词：

fluoxetine; norfluoxetine;

D O I：

10.1016/S1570-0232(01)00638-9

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A micellar electrokinetic capillary chromatography (MEKC) for determining fluoxetine and its metabolite (norfluoxetine) is proposed. Optimal conditions for the quantitative separation were investigated. A background electrolyte solution consisting of 5 mM phosphate buffer adjusted to pH 12.3 and 40 mM of 1-decanesulfonic acid sodium salt (DSS), hydrodynamic injection and 25 W of separation voltage were used. Good linearity and precision were obtained for both compounds. Detection limits of 0.2 mg/l for fluoxetine and norfluoxetine were obtained. The developed method is rapid and it has been applied to determine fluoxetine and its metabolite in human serum and urine. The samples were purified and enriched by means of extraction-preconcentration step with a preconditioned C,. cartridge and eluting the compounds with methanol. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：261 / 268

页数：8

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