Optimization study for the reversed-phase ion-pair liquid chromatographic determination of nicotine in commercial tobacco products

被引：38

作者：

Ciolino, LA ^{[1
]}

Turner, JA ^{[1
]}

McCauley, HA ^{[1
]}

Smallwood, AW ^{[1
]}

Yi, TY ^{[1
]}

机构：

[1] US FDA, Forens Chem Ctr, Cincinnati, OH 45202 USA

来源：

JOURNAL OF CHROMATOGRAPHY A | 1999年 / 852卷 / 02期

关键词：

tobacco; nicotine; alkaloids;

D O I：

10.1016/S0021-9673(99)00639-1

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The availability of published methods for the determination of nicotine in commercial tobacco products based on state-of-the-art chromatographic methods is limited. Nicotine is a diprotic base with pK(a)'s of 3.12 (pyridine ring) and 8.02 (pyrrolidine ring). Other monoprotic and diprotic bases are also present in commercial tobacco including anatabine, nornicotine, anabasine, and cotinine. In this paper, the chromatography of nicotine and the minor tobacco alkaloids under reversed-phase ion-pairing conditions is thoroughly studied. The results of this study are used to understand the retention mechanisms of the tobacco alkaloids, to examine their observed elution order with respect to fundamental analyte properties (size,functionality, and acid-base strength), and to select optimum chromatographic conditions for the determination of nicotine in commercial tobacco products. Published by Elsevier Science B.V.

引用

页码：451 / 463

页数：13

共 21 条

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