Purification, crystallization and preliminary structural characterization of human Rap1GAP

被引：4

作者：

Daumke, O ^{[1
]}

Wittinghofer, A ^{[1
]}

Weyand, M ^{[1
]}

机构：

[1] Max Planck Inst Mol Physiol, Abt Strukturbiol, D-44227 Dortmund, Germany

来源：

ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY | 2004年 / 60卷

关键词：

D O I：

10.1107/S0907444904002392

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Human Rap1GAP, the GTPase-activating protein (GAP) for the small GTPase Rap1, was recombinantly expressed in Escherichia coli, purified and crystallized using the hanging-drop vapour-diffusion method. Crystals were obtained using PEG 3350 as a precipitating agent and belong to the orthorhombic space group P2(1)2(1)2, with unit-cell parameters a=170.7, b=224.5, c=48.7 Angstrom. A complete data set was collected to 2.9 Angstrom resolution at 100 K using synchrotron radiation. The structure may reveal features of the unique reaction mechanism of Rap1GAP.

引用

页码：752 / 754

页数：3

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