The C-terminal region (640-967) of Arabidopsis CPL1 interacts with the abiotic stress- and ABA-responsive transcription factors

被引：18

作者：

Bang, Woo Young ^{[1
]}

Kim, Se Won ^{[1
]}

Jeong, In Sil ^{[1
]}

Koiwa, Hisashi ^{[2
]}

Bahk, Jeong Dong ^{[1
]}

机构：

[1] Gyeongsang Natl Univ, Grad Sch, Div Appl Life Sci BK21 EBNCRC, Jinju 660701, South Korea

[2] Texas A&M Univ, Dept Hort Sci, College Stn, TX 77843 USA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2008年 / 372卷 / 04期

基金：

美国国家科学基金会;

关键词：

AtCPL1; AtMYB3; ANAC019; CTD phosphatase; dsRNA-binding motif;

D O I：

10.1016/j.bbrc.2008.05.161

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Proteins in CPL1 family are unique to plants and contain a phosphatase catalytic domain and double-stranded RNA (dsRNA)-binding motifs (DRMs) in a single peptide. Though DRMs are important for the function of Arabidopsis CPL1 in vivo, the role of CPL1 DRM has been obscure. We have isolated two transcription factors, ANAC019 (Atlg52890) and AtMYB3 (Atlg22640), which specifically interact with the C-terminal region (640-967) of AtCPL1 containing two DRMs. Detailed interaction analysis indicated that AtMYB3 specifically interacted with the first DRM but not with the second DRM in CPL1 C-terminal fragment. GFP-fusion analysis indicated that AtMYB3 localized in nuclei-like CPL1, and its expression is induced by abiotic stress and ABA treatment. These results suggest that AtMYB3 function in abiotic stress signaling in concert with CPL1. (c) 2008 Elsevier Inc. All rights reserved.

引用

页码：907 / 912

页数：6

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