The nucleoprotein and the viral RNA of infectious salmon anemia virus (ISAV) are localized in the nucleolus of infected cells

被引：15

作者：

Goic, Bertsy ^{[1
]}

Bustamante, Juanita

Miquel, Alvaro

Alvarez, Marco ^{[1
]}

Vera, M. Ines ^{[1
]}

Valenzuela, Pablo D. T. ^{[1
]}

Burzio, Luis O. ^{[1
]}

机构：

[1] Univ Andres Bello, Dept Ciencias Biol, Fac Ciencias Salud, Santiago, Chile

来源：

VIROLOGY | 2008年 / 379卷 / 01期

关键词：

ISAV; nucleoprotein; nucleoli; nucleolin; in situ hybridization;

D O I：

10.1016/j.virol.2008.05.036

中图分类号：

Q93 [微生物学];

学科分类号：

071005 [微生物学]; 100705 [微生物与生化药学];

摘要：

The infectious salmon anemia Virus (ISAV), which belongs to the new genus Isavirus of the Orthomyxoviridae family, is an important pathogen of the salmon farming industry. Indirect immunofluorescence assays carried out with monoclonal antibodies specific for the nucleoprotein (NP) reveal differential staining of sub-cellular compartments in infected cells. Particularly interesting was the staining of the nucleolus, which showed co-localization with nucleolin in CHSE-214, EPC and SHK-1 cells infected with ISAV. These results were confirmed by co-immunoprecipitation studies showing an interaction between NP and nucleclin. In addition, in situ hybridization carried out with probes specific for each of the 8 RNA segments of ISAV showed that the genomic as well as the anti-genomic strands were also localized in the nucleolus. These results Suggest a role of the nucleolus in the replication and/or in the packaging of the ISAV genome. (C) 2008 Elsevier Inc. All rights reserved.

引用

页码：55 / 63

页数：9

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