Development of gene transfer methods for Rubrivivax gelatinosus S1: Construction, characterization and complementation of a puf operon deletion strain

被引：22

作者：

Ouchane, S ^{[1
]}

Picaud, M ^{[1
]}

ReissHusson, F ^{[1
]}

Vernotte, C ^{[1
]}

Astier, C ^{[1
]}

机构：

[1] CNRS, CTR GENET MOL, F-91198 GIF SUR YVETTE, FRANCE

来源：

MOLECULAR AND GENERAL GENETICS | 1996年 / 252卷 / 04期

关键词：

carotenoids; gene transfer; gene organization; photosynthetic bacteria; photosynthetic mutant;

D O I：

10.1007/BF02173002

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Gene transfer systems were developed in Rubrivivax (Rx.) gelatinosus S1. First, a system for conjugative transfer of mobilizable plasmids from Escherichia coli to Rx. gelatinosus S1 was established. Secondly, optimal conditions for the transformation of Rx. gelatinosus S1 by electroporation were determined. A Delta puf strain was constructed. Complementation with the puf operon from a wild-type strain cloned in a replicative plasmid restored photosynthetic growth. Two insertion strains were also selected. All the strains constructed were green, due to a change in carotenoid content. Characterization of these strains provides genetic evidence for a ''superoperon'' organization in this bacterium.

引用

页码：379 / 385

页数：7

共 33 条

[1] PURIFICATION AND CHARACTERIZATION OF RHODOCYCLUS-GELATINOSUS PHOTOCHEMICAL-REACTION CENTER [J].