Direct transfer of extended groups from synthetic cofactors by DNA methyltransferases

被引：185

作者：

Dalhoff, C

Lukinavicius, G

Klimasauskas, S

Weinhold, E

机构：

[1] Inst Biotechnol, Lab Biol DNA Modificat, LT-02241 Vilnius, Lithuania

[2] Rhein Westfal TH Aachen, Inst Organ Chem, D-52056 Aachen, Germany

来源：

NATURE CHEMICAL BIOLOGY | 2006年 / 2卷 / 01期

关键词：

D O I：

10.1038/nchembio754

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Adenosyl-L-methionine (AdoMet) is the major methyl donor for biological methylation reactions catalyzed by methyltransferases. We report the first chemical synthesis of AdoMet analogs with extended carbon chains replacing the methyl group and their evaluation as cofactors for all three classes of DNA methyltransferases. Extended groups containing a double or triple bond in the beta position to the sulfonium center were transferred onto DNA in a catalytic and sequence-specific manner, demonstrating a high utility of such synthetic cofactors for targeted functionalization of biopolymers.

引用

页码：31 / 32

页数：2

共 15 条

[1] POTENTIAL INHIBITORS OF S-ADENOSYLMETHIONINE-DEPENDENT METHYLTRANSFERASES .5. ROLE OF ASYMMETRIC SULFONIUM POLE IN ENZYMATIC BINDING OF S-ADENOSYL-L-METHIONINE [J].