Determination of cyanocobalamin, betamethasone, and diclofenac sodium in pharmaceutical formulations, by high performance liquid chromatography

被引：54

作者：

González, L ^{[1
]}

Yuln, G ^{[1
]}

Volonté, MG ^{[1
]}

机构：

[1] Natl Univ La Plata, Fac Ciencias Exactas, Dept Ciencias Biol, Div Farm,Catedra Esnayo & Valorac Medicamentos, RA-1900 La Plata, Argentina

来源：

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 1999年 / 20卷 / 03期

关键词：

cyanocobalamin; betamethasone; diclofenac sodium; high performance liquid chromatography; validation;

D O I：

10.1016/S0731-7085(99)00046-1

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The aim of this work was to develop an analytical method for a simultaneous determination of cyanocobalamin (Vitamin B12), betamethasone, and diclofenac, present in pharmaceutical formulations, by high performance liquid chromatography, assuring rapidity, accuracy, precision, and selectivity. The working conditions were as follows: RP18 column of 125 mm x 4 mm ID and a particle size of 5 mu m; mobile phase acetonitrile-water (40:60; v/v) (pH* 3.45) adjusted with acetic acid; flow gradient from 0.8 to 1.9 ml/min.; injection volume of 20 mu l; temperature 34 degrees C and detection at 240 nm. The method was adequately validated, and linearity, accuracy, as well as the system, method and interday precision, for each active principle, were determined. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：487 / 492

页数：6

共 23 条

[1] HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY OF WATER-SOLUBLE VITAMINS .2. SIMULTANEOUS DETERMINATIONS OF VITAMIN-B1 VITAMIN-B2, VITAMIN-B6, AND VITAMIN-B12 IN PHARMACEUTICAL PREPARATIONS [J].