Protein engineering of α-amylase for low pH performance

被引：68

作者：

Shaw, A ^{[1
]}

Bott, R ^{[1
]}

Day, AG ^{[1
]}

机构：

[1] Genencor Int Inc, Palo Alto, CA 94304 USA

来源：

CURRENT OPINION IN BIOTECHNOLOGY | 1999年 / 10卷 / 04期

关键词：

D O I：

10.1016/S0958-1669(99)80063-9

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Industrial-scale starch liquefaction is currently constrained to operating at pH 6.0 and above, as the enzyme used in the process, Bacillus licheniformis alpha-amylase, is unstable at lower pH under the conditions used. There is a need develop an enzyme that can operate at lower pH. Recent progress has been made in engineering the B. licheniformis enzyme for improved industrial performance. The availability of crystal structures and subsequent analysis of improved variants, in a structural context, is revealing common factors and a rationale to make further improvements.

引用

页码：349 / 352

页数：4

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