Neuromyelitis optica and anti-aquaporin-4 antibodies measured by an enzyme-linked immunosorbent assay

被引：93

作者：

Hayakawa, Sei ^{[1
]}

Mori, Masahiro ^{[1
]}

Okuta, Akiko ^{[2
]}

Kamegawa, Akiko ^{[3
,4
]}

Fujiyoshi, Yoshinori ^{[4
,5
]}

Yoshiyama, Yasumasa ^{[6
]}

Mitsuoka, Kaoru ^{[5
]}

Ishibashi, Kenichi ^{[7
]}

Sasaki, Sei ^{[8
]}

Hattori, Takamichi ^{[1
]}

Kuwabara, Satoshi ^{[1
]}

机构：

[1] Chiba Univ, Grad Sch Med, Dept Neurol, Chou Ku, Chiba 2608670, Japan

[2] JBIC, JBIRC, Tokyo, Japan

[3] Japan Sci & Technol Agcy, CREST, Kyoto, Japan

[4] Kyoto Univ, Fac Sci, Dept Biophys, Kyoto, Japan

[5] Natl Inst Adv Ind Sci & Technol, JBIRC, Tokyo, Japan

[6] Chiba E Natl Hosp, Clin Res Ctr, Dept Neurol, Chiba, Japan

[7] Meiji Pharmaceut Univ, Dept Med Physiol, Tokyo, Japan

[8] Tokyo Med & Dent Univ, Dept Nephrol, Tokyo, Japan

来源：

JOURNAL OF NEUROIMMUNOLOGY | 2008年 / 196卷 / 1-2期

关键词：

neuromyelitis optica; multiple sclerosis; aquaporin-4; anti-aquaporin-4; antibody; ELISA;

D O I：

10.1016/j.jneuroim.2008.03.009

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

NMO-IgG, a disease-specific autoantibody for neuromyelitis optica, recognizes aquaporin-4 (AQP4) and has been examined by indirect immunofluorescence assay. We developed an enzyme-linked immunosorbent assay (ELISA) to detect anti-AQP4 antibodies by establishing methods for expression in a baculovirus system and purification of recombinant AQP4 as antigen. Elevated anti-AQP4 antibody titers in serum were found in 15 (71%) of 21 patients with neuromyelitis optica, 4.3% of 46 patients with multiple sclerosis, none of 51 normal controls, and 2.6% of 115 patients with other neurological diseases. The ELISA system can be substituted for the conventional NMO-IgG assay. (C) 2008 Published by Elsevier B.V.

引用

页码：181 / 187

页数：7

共 27 条

[1] Brain magnetic resonance imaging findings in relapsing neuromyelitis optica [J].

Cabrera-Gomez, Jose A. ;

Quevedo-Sotolongo, L. ;

Gonzalez-Quevedo, A. ;

Lima, S. ;

Real-Gonzalez, Y. ;

Cristofol-Corominas, M. ;

Romero-Garcia, K. ;

Ugarte-Sanchez, C. ;

Jordan-Gonzalez, J. ;

Gonzalez de la Nuez, J. E. ;

Garcia Lahera, J. ;

Tellez, R. ;

Pedroso-Ibanez, I. ;

Rodriguez Roca, R. ;

Cabrera-Nunez, A. Y. .

MULTIPLE SCLEROSIS, 2007, 13 (02) :186-192

[2]

Devic E., 1894, Bull Med, V8, P1033

[3] Characterization of human endothelin B receptor and mutant receptors expressed in insect cells [J].

Doi, T ;

Hiroaki, Y ;

Arimoto, I ;

Fujiyoshi, Y ;

Okamoto, T ;

Satoh, M ;

Furuichi, Y .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1997, 248 (01) :139-148

[4] CLONING AND EXPRESSION OF APICAL MEMBRANE WATER CHANNEL OF RAT-KIDNEY COLLECTING TUBULE [J].

FUSHIMI, K ;

UCHIDA, S ;

HARA, Y ;

HIRATA, Y ;

MARUMO, F ;

SASAKI, S .

NATURE, 1993, 361 (6412) :549-552

[5] Pathogenic potential of IgG binding to water channel extracellular domain in neuromyelitis optica [J].

Hinson, S. R. ;

Pittock, S. J. ;

Lucchinetti, C. F. ;

Roemer, S. F. ;

Fryer, J. P. ;

Kryzer, T. J. ;

Lennon, V. A. .

NEUROLOGY, 2007, 69 (24) :2221-2231

[6] Implications of the aquaporin-4 structure on array formation and cell adhesion [J].

Hiroaki, Y ;

Tani, K ;

Kamegawa, A ;

Gyobu, N ;

Nishikawa, K ;

Suzuki, H ;

Walz, T ;

Sasaki, S ;

Mitsuoka, K ;

Kimura, K ;

Mizoguchi, A ;

Fujiyoshi, Y .

JOURNAL OF MOLECULAR BIOLOGY, 2006, 355 (04) :628-639

[7] MOLECULAR CHARACTERIZATION OF AN AQUAPORIN CDNA FROM BRAIN - CANDIDATE OSMORECEPTOR AND REGULATOR OF WATER-BALANCE [J].

JUNG, JS ;

BHAT, RV ;

PRESTON, GM ;

GUGGINO, WB ;

BARABAN, JM ;

AGRE, P .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (26) :13052-13056

[8] Multiple sclerosis in the Japanese population [J].

Kira, J .

LANCET NEUROLOGY, 2003, 2 (02) :117-127

[9]

Kira J, 1996, ANN NEUROL, V40, P569

[10] NATIONWIDE SURVEY OF MULTIPLE-SCLEROSIS IN JAPAN - CLINICAL ANALYSIS OF 1,084 CASES [J].

KUROIWA, Y ;

IGATA, A ;

ITAHARA, K ;

KOSHIJIMA, S ;

TSUBAKI, T ;

TOYOKURA, Y ;

SHIBASAKI, H .

NEUROLOGY, 1975, 25 (09) :845-851

← 1 2 3 →