Crystallographic studies on a family B DNA polymerase from hyperthermophilic archaeon Pyrococcus kodakaraensis strain KOD1

被引：15

作者：

Hashimoto, H

Matsumoto, T

Nishioka, M

Yuasa, T

Takeuchi, S

Inoue, T

Fujiwara, S

Takagi, M

Imanaka, T

Kai, Y ^{[1
]}

机构：

[1] Osaka Univ, Grad Sch Engn, Dept Chem Mat, Osaka 5650871, Japan

[2] Osaka Univ, Grad Sch Engn, Dept Biotechnol, Osaka 5650871, Japan

[3] Kyoto Univ, Grad Sch Engn, Dept Synthet Chem & Biol Chem, Sakyo Ku, Kyoto 6068501, Japan

来源：

JOURNAL OF BIOCHEMISTRY | 1999年 / 125卷 / 06期

关键词：

crystallization; family B DNA polymerase; hyperthermophilic archaeon; polymerase chain reaction; X-ray crystallography;

D O I：

10.1093/oxfordjournals.jbchem.a022405

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A hyperthermostable family B DNA polymerase from the hyperthermophilic archaeon, Pyrococcus kodakaraensis strain KOD1, has been crystallized by the hanging-drop vapor diffusion method at 293 K with 2-methyl-2,4-pentanediol as the precipitant. The diffraction pattern of a crystal extends to 3.0 Angstrom resolution, and two full sets of 3.0 Angstrom resolution diffraction data for native crystals were successfully collected at 290 K and 100 K upon exposure to synchrotron radiation at KEK-PF, Japan. The crystals belong to the space group, P2(1)2(1)2(1), with unit-cell dimensions of a=112.8, b=115.4, and c =75.4 A at 290 K, and a=111.9, b=112.4, and c=73.9 at 100 K, Structural analysis by means of the multiple isomorphous replacement method is now in progress.

引用

页码：983 / 986

页数：4

共 19 条

[1] STRUCTURAL AND FUNCTIONAL-RELATIONSHIPS BETWEEN PROKARYOTIC AND EUKARYOTIC DNA-POLYMERASES [J].