Molecular typing and characterization of extended-spectrum TEM, SHV and CTX-M β-lactamases in clinical isolates of Enterobacter cloacae

被引：15

作者：

Biendo, Maurice ^{[1
]}

Manoliu, Claudia ^{[1
]}

Laurans, Genevieve ^{[1
]}

Castelain, Sandrine ^{[2
]}

Canarelli, Brigitte ^{[1
]}

Thomas, Daniele ^{[1
]}

Hamdad, Farida ^{[1
]}

Rousseau, Florence ^{[1
]}

Eb, Francois ^{[1
]}

机构：

[1] CHU Nord, Serv Bacteriol, F-80054 Amiens 1, France

[2] Fac Med, Virol Lab, F-80036 Amiens, France

来源：

RESEARCH IN MICROBIOLOGY | 2008年 / 159卷 / 9-10期

关键词：

Enterobacter cloacae; Extended-spectrum beta-lactamases; Pulsed-field gel electrophoresis; Polymerase chain reactions; TEM; SHV; CTX-M;

D O I：

10.1016/j.resmic.2008.09.003

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Sixty-one non-repetitive Enterobacter cloacae ESBL producers were collected at the Amiens University Hospital in France. Eight beta-lactam resistance phenotypes (a-h) and three aminoglycoside resistance phenotypes (i-k) were identified among these isolates, and 32 different pulsotypes were observed. Of these 61 isolates, 37 were sequenced and found to harbor beta-lactamases with a pl of 5.9 (TEM-4), 6.5 (TEM-24), 7.8 (SHV-4), 8.2 (SHV-12), 8.4 (CTX-M-1) and 8.0 (CTX-M-9). Four imipenem-resistant ESBL-producing E. cloacae isolates did not express the 38 kDa OMP, indicating that this resistance is associated with porin deficiency. (C) 2008 Elsevier Masson SAS. All fights reserved.

引用

页码：590 / 594

页数：5

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