Cloning and characterization of the Streptomyces peucetius dnmZUV genes encoding three enzymes required for biosynthesis of the daunorubicin precursor thymidine diphospho-L-daunosamine

被引：52

作者：

Otten, SL

Gallo, MA

Madduri, K

Liu, XC

Hutchinson, CR

机构：

[1] UNIV WISCONSIN,SCH PHARM,MADISON,WI 53706

[2] UNIV WISCONSIN,DEPT BACTERIOL,MADISON,WI 53706

来源：

JOURNAL OF BACTERIOLOGY | 1997年 / 179卷 / 13期

关键词：

D O I：

10.1128/jb.179.13.4446-4450.1997

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Characterization of the dnmZ, dnmU, and dnmV genes from the daunorubicin-producer Streptomyces peucetius by DNA sequence analysis indicated that these genes encode a protein of unknown function plus a putative thymidine diphospho-1-keto-6-deoxyglucose-3(5)-epimerase and thymidine diphospho-1-ketodeoxyhexulose reductase, respectively. Inactivation of each of the three genes by gene disruption and replacement in the wild-type strain demonstrated that all of them are required for daunosamine biosynthesis.

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页码：4446 / 4450

页数：5

相关论文

共 33 条

[1]

ALTSCHUL SF, 1990, J MOL BIOL, V215, P403, DOI 10.1006/jmbi.1990.9999

[2] TIGHTLY REGULATED TAC PROMOTER VECTORS USEFUL FOR THE EXPRESSION OF UNFUSED AND FUSED PROTEINS IN ESCHERICHIA-COLI [J].

AMANN, E ;

OCHS, B ;

ABEL, KJ .

GENE, 1988, 69 (02) :301-315

[3] PLASMID CLONING VECTORS FOR THE CONJUGAL TRANSFER OF DNA FROM ESCHERICHIA-COLI TO STREPTOMYCES SPP [J].

BIERMAN, M ;

LOGAN, R ;

OBRIEN, K ;

SENO, ET ;

RAO, RN ;

SCHONER, BE .

GENE, 1992, 116 (01) :43-49

[4] MOLECULAR AND BIOCHEMICAL ANALYSES OF FATTY-ACID TRANSPORT, METABOLISM, AND GENE-REGULATION IN ESCHERICHIA-COLI [J].

BLACK, PN ;

DIRUSSO, CC .

BIOCHIMICA ET BIOPHYSICA ACTA-LIPIDS AND LIPID METABOLISM, 1994, 1210 (02) :123-145

[5] THE RHIZOBIUM-MELILOTI EXOZL EXOB FRAGMENT OF MEGAPLASMID-2 - EXOB FUNCTIONS AS A UDP-GLUCOSE 4-EPIMERASE AND EXOZ SHOWS HOMOLOGY TO NODX OF RHIZOBIUM-LEGUMINOSARUM BIOVAR VICIAE STRAIN TOM [J].

BUENDIA, AM ;

ENENKEL, B ;

KOPLIN, R ;

NIEHAUS, K ;

ARNOLD, W ;

PUHLER, A .

MOLECULAR MICROBIOLOGY, 1991, 5 (06) :1519-1530

[6] A COMPREHENSIVE SET OF SEQUENCE-ANALYSIS PROGRAMS FOR THE VAX [J].

DEVEREUX, J ;

HAEBERLI, P ;

SMITHIES, O .

NUCLEIC ACIDS RESEARCH, 1984, 12 (01) :387-395

[7]

FUJIWARA A, 1983, CRC CRITICAL REV BIO, V3, P133

[8] The dnrM gene in Streptomyces peucetius contains a naturally occurring frameshift mutation that is suppressed by another locus outside of the daunorubicin-production gene cluster [J].

Gallo, MA ;

Ward, J ;

Hutchinson, CR .

MICROBIOLOGY-UK, 1996, 142 :269-275

[9] A BACTERIAL ANALOG OF THE MDR GENE OF MAMMALIAN TUMOR-CELLS IS PRESENT IN STREPTOMYCES-PEUCETIUS, THE PRODUCER OF DAUNORUBICIN AND DOXORUBICIN [J].

GUILFOILE, PG ;

HUTCHINSON, CR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (19) :8553-8557

[10] GENE DISRUPTION AND GENE REPLACEMENT IN STREPTOMYCES VIA SINGLE-STRANDED-DNA TRANSFORMATION OF INTEGRATION VECTORS [J].

HILLEMANN, D ;

PUHLER, A ;

WOHLLEBEN, W .

NUCLEIC ACIDS RESEARCH, 1991, 19 (04) :727-731

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