An enzyme controlled by light: the molecular mechanism of photoreactivity in nitrile hydratase

被引：73

作者：

Endo, I ^{[1
]}

Odaka, M ^{[1
]}

Yohda, M ^{[1
]}

机构：

[1] Inst Phys & Chem Res, Biochem Syst Lab, Wako, Saitama 3510198, Japan

来源：

TRENDS IN BIOTECHNOLOGY | 1999年 / 17卷 / 06期

关键词：

D O I：

10.1016/S0167-7799(99)01303-7

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Extensive studies have revealed the molecular mechanism of the photoreactivity of nitrile hydratase from Rhodococcus sp, N-771. In the inactive enzyme, nitric oxide is bound to the non-heme ferric iron at the catalytic center, stabilized by a claw like structure formed by two post-translationally modified cysteines and a serine. The inactive nitrile hydratase is activated by the photoinduced release of the nitric oxide. This result might provide a means of designing novel photoreactive chemical compounds or proteins that would be applicable to biochips and light-controlled metabolic systems.

引用

页码：244 / 249

页数：6

共 47 条

[1] REDOX REGULATION OF FOS AND JUN DNA-BINDING ACTIVITY INVITRO [J].

ABATE, C ;

PATEL, L ;

RAUSCHER, FJ ;

CURRAN, T .

SCIENCE, 1990, 249 (4973) :1157-1161

[2]

AKAIKE T, 1996, METHOD ENZYMOL, V268, P221

[3]

BANDYOPADHYAY S, 1994, J BIOL CHEM, V269, P29949

[4] Highly efficient control of iron-containing nitrile hydratases by stoichiometric amounts of nitric oxide and light [J].

Bonnet, D ;

Artaud, I ;

Moali, C ;

Petre, D ;

Mansuy, D .

FEBS LETTERS, 1997, 409 (02) :216-220

[5] Resonance Raman spectroscopy of nitrile hydratase, a novel iron - Sulfur enzyme [J].

Brennan, BA ;

Cummings, JG ;

Chase, DB ;

Turner, IM ;

Nelson, MJ .

BIOCHEMISTRY, 1996, 35 (31) :10068-10077

[6] Nitrile hydratase from Rhodococcus rhodochrous J1 contains a non-corrin cobalt ion with two sulfur ligands [J].

Brennan, BA ;

Alms, G ;

Nelson, MJ ;

Durney, LT ;

Scarrow, RC .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1996, 118 (38) :9194-9195

[7] PROTEIN-SULFENIC ACID STABILIZATION AND FUNCTION IN ENZYME CATALYSIS AND GENE-REGULATION [J].

CLAIBORNE, A ;

MILLER, H ;

PARSONAGE, D ;

ROSS, RP .

FASEB JOURNAL, 1993, 7 (15) :1483-1490

[8] REACTION OF NITRIC-OXIDE WITH THE FREE SULFHYDRYL-GROUP OF HUMAN SERUM-ALBUMIN YIELDS A SULFENIC ACID AND NITROUS-OXIDE [J].

DEMASTER, EG ;

QUAST, BJ ;

REDFERN, B ;

NAGASAWA, HT .

BIOCHEMISTRY, 1995, 34 (36) :11494-11499

[9] An implicit TRIPLE effect in Mims pulsed ENDOR: A sensitive new technique for determining signs of hyperfine couplings [J].

Doan, PE ;

Nelson, MJ ;

Jin, HY ;

Hoffmann, BM .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1996, 118 (29) :7014-7015

[10] SPECTROSCOPIC OBSERVATION OF THE INTRAMOLECULAR ELECTRON-TRANSFER IN THE PHOTOACTIVATION PROCESSES OF NITRILE HYDRATASE [J].

HONDA, J ;

KANDORI, H ;

OKADA, T ;

NAGAMUNE, T ;

SHICHIDA, Y ;

SASABE, H ;

ENDO, I .

BIOCHEMISTRY, 1994, 33 (12) :3577-3583

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