Measure of carbon and nitrogen stable isotope ratios in cultured cells

被引:70
作者
Peteranderl, R
Lechene, C
机构
[1] Brigham & Womens Hosp, Dept Med, Boston, MA 02115 USA
[2] Harvard Univ, Sch Med, Boston, MA 02115 USA
关键词
D O I
10.1016/j.jasms.2003.11.019
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
We report the measurement of the natural isotope ratios of nitrogen and carbon in subcellular volumes of individual cells among a population of cultured cells using a multi-isotope imaging mass spectrometer (MIMS), [MIMS is the prototype of the NanoSIMS 50, Cameca, France.] We also measured the nitrogen and carbon isotope ratio in cells after they had been cultured in media enriched with the amino acid glycine labeled with either C-13 or N-15. The results demonstrate that C-13/C-12 and N-15/N-14 isotope ratios can be measured directly on a subcellular scale. This opens the way for the use of stable isotopes, in particular N-15, as labels to measure the intracellular turnover of biomolecules. Such a capability should help resolve a wide range of biomedical problems. (C) 2004 American Society for Mass Spectrometry.
引用
收藏
页码:478 / 485
页数:8
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