Characterization of Astragalus sinicus rhizobia by restriction fragment length polymorphism analysis of chromosomal and nodulation genes regions

被引：18

作者：

Guo, XW ^{[1
]}

Zhang, XX ^{[1
]}

Zhang, ZM ^{[1
]}

Li, FD ^{[1
]}

机构：

[1] Huazhong Agr Univ, Dept Microbiol, Wuhan 430070, Hubei, Peoples R China

来源：

CURRENT MICROBIOLOGY | 1999年 / 39卷 / 06期

关键词：

D O I：

10.1007/s002849900472

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Two hundred and four isolates of rhizobia were sampled from root nodules of Astragalus sinicus grown in rice gelds of six southern provinces of China. Genotypic diversity was determined by Southern hybridization using nodDBC genes as a probe, restriction fragment length polymorphism (RFLP) analysis of PCR-amplified 16S-23S rDNA intergenic spacers (IGS), and plasmid profile. Our results show that rhizobia associated with A. sinicus were very diverse, and 10 genotypes were resolved within the previously identified dominant 16S rDNA type. Diversity levels varied greatly between different geographical locations. The same nod gene genotypes were harbored by distinct chromosomal types, suggesting that lateral plasmid transfer occurred during the evolution process.

引用

页码：358 / 364

页数：7

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