Engaging the ribosome: universal IFs of translation

被引：61

作者：

Roll-Mecak, A

Shin, BS

Dever, TE

Burley, SK

机构：

[1] Rockefeller Univ, Howard Hughes Med Inst, Lab Mol Biophys, New York, NY 10021 USA

[2] NICHHD, Lab Gene Regulat & Dev, NIH, Bethesda, MD 20892 USA

来源：

TRENDS IN BIOCHEMICAL SCIENCES | 2001年 / 26卷 / 12期

关键词：

D O I：

10.1016/S0968-0004(01)02024-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Eukaryotic initiation factor 1A (eIF1A) and the GTPase IF2/eIF5B are the only universally conserved translation initiation factors. Recent structural, biochemical and genetic data indicate that these two factors form an evolutionarily conserved structural and functional unit in translation initiation. Based on insights gathered from studies of the translation elongation factor GTPases, we propose that these factors occupy the aminoacyl-tRNA site (A site) on the ribosome, and promote initiator tRNA binding and ribosomal subunit joining. These processes yield a translationally competent ribosome with Met-tRNA in the ribosomal peptidyl-tRNA site (P site), base-paired to the AUG start codon of a mRNA.

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收藏

页码：705 / 709

页数：5

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