Distinct mesoderm migration phenotypes in extra-embryonic and embryonic regions of the early mouse embryo

被引：63

作者：

Saykali, Bechara ^{[1
]}

Mathiah, Navrita ^{[1
]}

Nahaboo, Wallis ^{[1
]}

Racu, Marie-Lucie ^{[1
]}

Hammou, Latifa ^{[1
]}

Defrance, Matthieu ^{[2
]}

Migeotte, Isabelle ^{[1
,3
]}

机构：

[1] Univ Libre Bruxelles, IRIBHM, Brussels, Belgium

[2] Univ Libre Bruxelles, Interuniv Inst Bioinformat Brussels, Brussels, Belgium

[3] Walloon Excellence Lifesci & Biotechnol, Wallonia, Belgium

来源：

ELIFE | 2019年 / 8卷

关键词：

KERATIN INTERMEDIATE-FILAMENTS; RECEPTOR TYROSINE KINASE; CELL-MIGRATION; MESENCHYMAL TRANSITION; EXPRESSION ANALYSIS; RHO GTPASES; GERM LAYER; TISSUE; GASTRULATION; ROLES;

D O I：

10.7554/eLife.42434

中图分类号：

Q [生物科学];

学科分类号：

090105 [作物生产系统与生态工程];

摘要：

In mouse embryo gastrulation, epiblast cells delaminate at the primitive streak to form mesoderm and definitive endoderm, through an epithelial-mesenchymal transition. Mosaic expression of a membrane reporter in nascent mesoderm enabled recording cell shape and trajectory through live imaging. Upon leaving the streak, cells changed shape and extended protrusions of distinct size and abundance depending on the neighboring germ layer, as well as the region of the embryo. Embryonic trajectories were meandrous but directional, while extra-embryonic mesoderm cells showed little net displacement. Embryonic and extra-embryonic mesoderm transcriptomes highlighted distinct guidance, cytoskeleton, adhesion, and extracellular matrix signatures. Specifically, intermediate filaments were highly expressed in extra-embryonic mesoderm, while live imaging for F-actin showed abundance of actin filaments in embryonic mesoderm only. Accordingly, Rhoa or Rac1 conditional deletion in mesoderm inhibited embryonic, but not extra-embryonic mesoderm migration. Overall, this indicates separate cytoskeleton regulation coordinating the morphology and migration of mesoderm subpopulations.

引用

页数：27

共 70 条

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