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Mono and dithiol surfaces on surface plasmon resonance biosensors for detection of Staphylococcus aureus

被引：85
作者：
Subramanian, A
Irudayaraj, J
Ryan, T
机构：
[1] Purdue Univ, W Lafayette, IN 47907 USA
[2] Penn State Univ, Dept Agr & Biol Engn, University Pk, PA 16802 USA
[3] Reichert Inc, Depew, NY 14043 USA
来源：
SENSORS AND ACTUATORS B-CHEMICAL | 2006年 / 114卷 / 01期
关键词：
surface plasmon resonance; Staphylococcus aureus; pathogen detection; monothiols; dithiols; self-assembled monolayers;
D O I：
10.1016/j.snb.2005.04.030
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
The capability of alkane monothiol and dithiol dendritic tether based self-assembled monolayers (SAM) in conjunction with surface plasmon resonance (SPR) for the detection of Staphylococcus aureus was investigated. An antibody based detection protocol for S. aureus, suspended in phosphate buffered saline with Tween 20 (PBST) was developed. Direct and sandwich assays were carried out on the immobilized antibodies against S. aureus. The effect of primary and secondary antibody concentrations on detection was investigated using a Box-Behnken surface response model. Specificity of anti-S. aureus to detect S. aureus in a mixture with Escherichia coli O157:H7 was also researched. Both the surfaces could detect S. aureus to the level of 10(5) CFU/ml in a sandwich assay, with good specificity against E. coli O157:H7. Results indicate that mono and dithiol self-assembled monolayers can be employed for rapid detection of S. aureus. (c) 2005 Elsevier B.V. All rights reserved.
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页码：192 / 198
页数：7
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