Endomorphins delay constitutive apoptosis and alter the innate host defense functions of neutrophils

被引:17
作者
Azuma, Y [1 ]
Ohura, K [1 ]
Wang, PL [1 ]
Shinohara, M [1 ]
机构
[1] Osaka Dent Univ, Dept Pharmacol, Hirakata, Osaka 5731121, Japan
基金
日本学术振兴会;
关键词
neutrophils; apoptosis; endomorphins; signal transduction; innate immune functions;
D O I
10.1016/S0165-2478(01)00335-2
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Recent studies have shown that opioid peptides are released from cells of the immune system during inflammation and stress, and are associated with altered immune responses. Moreover, concentrations of opioid peptides are increased in peripheral blood and at the sites of inflammatory reactions. The aim of this study was to evaluate immunological effects of opioid peptides endomorphins 1 and 2 on constitutive apoptosis. Superoxide anion production. hydrogen peroxide production, adhesion. phagocytosis. and chemotaxis of neutrophils. Neutrophils were isolated by peritoneal lavage from rats. Endomorphins 1 and 2 significantly delayed constitutive neutrophil apoptosis. The delay of neutrophil apoptosis was markedly attenuated by LY294002. a phosphoinositide 3-kinase inhibitor, Moreover, endomorphins 1 and 2 activated the phosphoinositide 3-kinase path ay as determined by phosphorylation of BAD. In contrast. endomorphins 1 and 2 blocked the production of superoxide anion and hydrogen peroxide by PMA-stimulated neutrophils. In addition. endomorphins 1 and 2 inhibited neutrophil adhesion to fibronectin. Moreover, endomorphins 1 and 2 potentiated neutrophil chemotaxis toward zymosan-activated serum and IL-8. respectively. However, endomorphins 1 and 2 did not alter phagocytosis of Escherichia coli by neutrophils. These results Suggest that endomorphins 1 and 2 may act to delay neutrophil apoptosis and alter the natural immune functions of neutrophils. (C) 2002 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:31 / 40
页数:10
相关论文
共 51 条
[1]   Immunomodulation of the neutrophil respiratory burst by endomorphins 1 and 2 [J].
Azuma, Y ;
Wang, PL ;
Shinohara, M ;
Ohura, K .
IMMUNOLOGY LETTERS, 2000, 75 (01) :55-59
[2]   Comparative studies of modulatory effect to the function of rat peritoneal neutrophils treated with new quinolones [J].
Azuma, Y ;
Wang, PL ;
Shinohara, M ;
Okamura, M ;
Inui, Y ;
Suese, Y ;
Ohura, K .
IMMUNOLOGY LETTERS, 1999, 69 (03) :321-327
[3]   Possible interaction between new quinolones and immune functions in macrophages [J].
Azuma, Y ;
Shinohara, M ;
Murakawa, N ;
Endo, M ;
Ohura, K .
GENERAL PHARMACOLOGY, 1999, 32 (05) :609-614
[4]   Comparison of inhibitory effects of local anesthetics on immune functions of neutrophils [J].
Azuma, Y ;
Shinohara, M ;
Wang, PL ;
Suese, Y ;
Yasuda, H ;
Ohura, K .
INTERNATIONAL JOURNAL OF IMMUNOPHARMACOLOGY, 2000, 22 (10) :789-796
[5]   Constitutive expression of cytoplasmic activator protein-1 with DNA binding activity and responsiveness to ionotropic glutamate signals in the murine hippocampus [J].
Azuma, Y ;
Ogita, K ;
Yoneda, Y .
NEUROSCIENCE, 1999, 92 (04) :1295-1308
[6]   Possible in vivo crosstalk between transcription factors with zinc-finger and leucine-zipper motifs in murine peripheral but not central excitable tissues [J].
Azuma, Y ;
Nishiguchi, M ;
Tagami, H ;
Ogita, K ;
Yoneda, Y .
NEUROCHEMISTRY INTERNATIONAL, 1998, 32 (04) :325-336
[7]   Differentiation by in vitro treatment of lidocaine-epinephrine and prilocaine-felypressine in neutrophils [J].
Azuma, Y ;
Wang, PL ;
Shinohara, M ;
Ohura, K .
IMMUNOLOGY LETTERS, 2001, 77 (03) :151-158
[8]   Endomorphins 1 and 2 modulate chemotaxis, phagocytosis and superoxide anion production by microglia [J].
Azuma, Y ;
Ohura, K ;
Wang, PL ;
Shinohara, M .
JOURNAL OF NEUROIMMUNOLOGY, 2001, 119 (01) :51-56
[9]   Histamine inhibits chemotaxis, phagocytosis, superoxide anion production, and the production of TNFα and IL-12 by macrophages via H2-receptors [J].
Azuma, Y ;
Shinohara, M ;
Wang, PL ;
Hidaka, A ;
Ohura, K .
INTERNATIONAL IMMUNOPHARMACOLOGY, 2001, 1 (9-10) :1867-1875
[10]   Quinolones alter defense reactions mediated by macrophages [J].
Azuma, Y ;
Shinohara, M ;
Wang, PL ;
Ohura, K .
INTERNATIONAL IMMUNOPHARMACOLOGY, 2001, 1 (02) :179-187