Persistence length of single-stranded DNA

被引：475

作者：

Tinland, B

Pluen, A

Sturm, J

Weill, G

机构：

[1] Institut Charles Sadron, CNRS, Université Louis Pasteur, 67083 Strasbourg, 6, rue Boussingault

来源：

MACROMOLECULES | 1997年 / 30卷 / 19期

关键词：

D O I：

10.1021/ma970381+

中图分类号：

O63 [高分子化学（高聚物）];

学科分类号：

070305 ; 080501 ; 081704 ;

摘要：

The self-diffusion coefficient of a series of DNA fragments ranging from 280 to 5386 bases has been measured by fluorescence recovery after photobleaching after thermal denaturation in 8 M urea. The total persistence length p of single-stranded DNAs and its variation in ionic strength down to 10(-3) M has been deduced. The importance of the value of p Versus the pore size a and contour length L of the DNA in the optimization of sequencing by gel electrophoresis is emphasized.

引用

收藏

页码：5763 / 5765

页数：3

相关论文

共 15 条

[1]

ACKSASU AZ, 1979, MACROMOLECULES, V12, P276

[2] PERSISTENCE LENGTH OF POLYELECTROLYTE CHAINS [J].

BARRAT, JL ;

JOANNY, JF .

EUROPHYSICS LETTERS, 1993, 24 (05) :333-338

[3] MEASUREMENT OF THE POROUS MICROSTRUCTURE OF HYDROGELS BY NUCLEAR-MAGNETIC-RESONANCE [J].

CHUI, MM ;

PHILLIPS, RJ ;

MCCARTHY, MJ .

JOURNAL OF COLLOID AND INTERFACE SCIENCE, 1995, 174 (02) :336-344

[4] FRINGE PATTERN PHOTOBLEACHING, A NEW METHOD FOR THE MEASUREMENT OF TRANSPORT-COEFFICIENTS OF BIOLOGICAL MACROMOLECULES [J].

DAVOUST, J ;

DEVAUX, PF ;

LEGER, L .

EMBO JOURNAL, 1982, 1 (10) :1233-1238

[5] MOBILITY OF A REPTATING POLYMER [J].

DUKE, TAJ ;

SEMENOV, AN ;

VIOVY, JL .

PHYSICAL REVIEW LETTERS, 1992, 69 (22) :3260-3263

[6]

KLENOWTABOR R, 1987, P NATL ACAD SCI USA, V84, P4767

[7] THEORY OF GEL-ELECTROPHORESIS OF DNA [J].

LUMPKIN, OJ ;

DEJARDIN, P ;

ZIMM, BH .

BIOPOLYMERS, 1985, 24 (08) :1573-1593

[8] POLYELECTROLYTES NEAR THE ROD LIMIT [J].

ODIJK, T .

JOURNAL OF POLYMER SCIENCE PART B-POLYMER PHYSICS, 1977, 15 (03) :477-483

[9]

PERNODET N, 1996, THESIS STRASBOURG

[10] THERMODYNAMIC ANALYSIS OF ION EFFECTS ON BINDING AND CONFORMATIONAL EQUILIBRIA OF PROTEINS AND NUCLEIC-ACIDS - ROLES OF ION ASSOCIATION OR RELEASE, SCREENING, AND ION EFFECTS ON WATER ACTIVITY [J].

RECORD, MT ;

ANDERSON, CF ;

LOHMAN, TM .

QUARTERLY REVIEWS OF BIOPHYSICS, 1978, 11 (02) :103-178