Generation of a chemical gradient across an array of 256 cell cultures in a single chip

被引：18

作者：

Somaweera, Himali ^{[1
]}

Ibragimov, Akif ^{[2
]}

Pappas, Dimitri ^{[1
,3
]}

机构：

[1] Texas Tech Univ, Dept Chem & Biochem, Lubbock, TX 79409 USA

[2] Texas Tech Univ, Dept Math & Stat, Lubbock, TX 79409 USA

[3] Texas Tech Univ, Ctr Chem Biol, Lubbock, TX 79409 USA

来源：

ANALYST | 2013年 / 138卷 / 19期

基金：

美国国家卫生研究院;

关键词：

MICROFLUIDIC DEVICE; TEMPORAL GRADIENTS; APOPTOSIS; SYSTEMS;

D O I：

10.1039/c3an00946g

中图分类号：

O65 [分析化学];

学科分类号：

070302 [分析化学];

摘要：

A microfluidic diffusion diluter to create stable chemical gradients across an array of cell cultures was demonstrated. The device enabled concentration based studies to be conducted at 256 different concentrations across individual, low shear cell cultures. A gradient of staurosporine on cells stained with Mitotracker Deep Red (MTDR) showed a concentration-based effect on cell apoptosis across the cell culture array.

引用

页码：5566 / 5571

页数：6

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