Apoptotic cell death in suspension cultures of Taxus chinensis var. mairei

被引:9
作者
Ma, ZY [1 ]
Yuan, YJ [1 ]
Wu, JC [1 ]
Zeng, AP [1 ]
机构
[1] Tianjin Univ, Sch Chem Engn & Technol, Dept Pharmaceut Engn, Tianjin 300072, Peoples R China
基金
中国国家自然科学基金;
关键词
apoptosis; suspension culture; taxol; Taxus chinensis var. mairei;
D O I
10.1023/A:1014820810258
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Apoptotic cell death was observed in suspension cultures of Taxus chinensis var. mairei under normal cultivation conditions by using microscopy, total DNA agarose gel electrophoresis and in situ end-labeling of fragmented DNA. The morphological and biochemical changes of cells occurred mainly in the non-dividing cell clusters, indicating that the T. chinensis cells died mainly by apoptosis. There exists a close relationship between cell apoptosis and Taxol formation. Taxol concentration increased with the increase in content of apoptotic cells and reached a maximum (14.2 mg l(-1)) after 23 days of culture, corresponding to a maximum ratio of apoptotic to total cells of about 13%.
引用
收藏
页码:573 / 577
页数:5
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