Reduced basal CaMKII levels in hippocampal CA1 region: Possible cause of stress-induced impairment of LTP in chronically stressed rats

被引:80
作者
Gerges, NZ [1 ]
Aleisa, AM [1 ]
Schwarz, LA [1 ]
Alkadhi, KA [1 ]
机构
[1] Univ Houston, Dept Pharmacol & Pharmaceut Sci, Coll Pharm, Houston, TX 77204 USA
关键词
long-term potentiation; chronic psychosocial stress; CaMKII; hippocampus; CA1; region; in vivo stimulation; PKC; calcineurin;
D O I
10.1002/hipo.10193
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Chronic psychosocial stress markedly reduces the expression of high-frequency stimulation (HFS)-evoked early long-term potentiation (LTP) in the CA1 region of the hippocampus of anesthetized rats. Immunoblotting was performed to determine changes in molecular levels of key signaling proteins that might be responsible for this inhibitory effect. Western blot analysis of the CA1 region demonstrates that chronic psychosocial stress decreases basal levels of calcium calmodulin kinase II (CaMKII), phosphorylated (P)-CaMKII, calmodulin, and protein kinase C (PKCgamma) while markedly increasing protein phosphatase 2B (calcineurin) levels. The decrease of basal levels of P-CaMKII may be triggered primarily by excessive dephosphorylation resulting from enhanced basal levels of calcineurin. The decline in the basal levels of the upstream molecules, PKCgamma and calmodulin may be a consequence of the diminished basal P-CaMKII levels. Analysis of signaling molecules in CA1 region of chronically stressed rat subjected to HFS in vivo showed only one difference compared to similarly stimulated control rats; no increase in P-CaMKII levels. Our results suggest that decreased P-CaMKI I levels may be primarily responsible for the stress-induced reduction in LTP expression. (C) 2004 Wiley-Liss, Inc.
引用
收藏
页码:402 / 410
页数:9
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