Aldehyde dehydrogenase 1A1 and gelsolin identified as novel invasion-modulating factors in conditioned medium of pancreatic cancer cells

被引：18

作者：

Walsh, Naomi ^{[1
]}

Dowling, Paul ^{[1
]}

O'Donovan, Norma ^{[1
]}

Henry, Michael ^{[1
]}

Meleady, Paula ^{[1
]}

Clynes, Martin ^{[1
]}

机构：

[1] Dublin City Univ, Natl Inst Cellular Biotechnol, Dublin 9, Ireland

来源：

JOURNAL OF PROTEOMICS | 2008年 / 71卷 / 05期

关键词：

ALDH1A1; GSN; Pancreatic cancer; Invasion; Conditioned media; 2D DIGE MALDI-TOF MS;

D O I：

10.1016/j.jprot.2008.09.002

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Conditioned medium (CM) from clonal sub-populations of the pancreatic cancer cell line, MiaPaCa-2 with differing invasive abilities, were examined for their effect on in vitro invasion. Conditioned medium from Clone #3 (CM#3) strongly promoted invasion, while CM from Clone #8 (CM#8) inhibited invasion in vitro. 2D DIGE followed by MALDI-TOF MS analysis of CM#3 and CM#8 identified 41 proteins which were differentially regulated; 27 proteins were down-regulated and 14 proteins up-regulated in the invasion-promoting CM#3 when compared to CM#8. Western blotting analysis confirmed the down-regulated expression of gelsolin and the up-regulation of aldehyde dehydrogenase 1A1 in CM#3. Down-regulation of aldehyde dehydrogenase 1A1 in Clone #3 CM and gelsolin levels in Clone #8 CM by siRNA transfection revealed an important involvement of these proteins in promoting and inhibiting invasion in these pancreatic cancer cell lines. (C) 2008 Elsevier B.V. All rights reserved.

引用

页码：561 / 571

页数：11

共 49 条

[1] A novel experimental design for comparative two-dimensional gel analysis: Two-dimensional difference gel electrophoresis incorporating a pooled internal standard [J].

Alban, A ;

David, SO ;

Bjorkesten, L ;

Andersson, C ;

Sloge, E ;

Lewis, S ;

Currie, I .

PROTEOMICS, 2003, 3 (01) :36-44

[2]

ALBINI A, 1987, CANCER RES, V47, P3239

[3]

Altmeyer A, 1996, INT J CANCER, V69, P340, DOI 10.1002/(SICI)1097-0215(19960822)69:4<340::AID-IJC18>3.0.CO

[4]

2-9

[5] Feature-based prediction of non-classical and leaderless protein secretion [J].

Bendtsen, JD ;

Jensen, LJ ;

Blom, N ;

von Heijne, G ;

Brunak, S .

PROTEIN ENGINEERING DESIGN & SELECTION, 2004, 17 (04) :349-356

[6] Gene array profiles of alcohol and aldehyde metabolizing enzymes in brains of C57BL/6 and DBA/2 mice [J].

Bhave, Sanjiv V. ;

Hoffman, Paula L. ;

Lassen, Natalie ;

Vasiliou, Vasilis ;

Saba, Laura ;

Deitrich, Richard A. ;

Tabakoff, Boris .

ALCOHOLISM-CLINICAL AND EXPERIMENTAL RESEARCH, 2006, 30 (10) :1659-1669

[7] ACTIN-DESTABILIZING FACTOR IS PRESENT IN HUMAN-PLASMA [J].

CHAPONNIER, C ;

BORGIA, R ;

RUNGGERBRANDLE, E ;

WEIL, R ;

GABBIANI, G .

EXPERIENTIA, 1979, 35 (08) :1039-1041

[8] Identification of 3-deoxyglucosone dehydrogenase as aldehyde dehydrogenase 1A1 (retinaldehyde dehydrogenase 1) [J].

Collard, Francois ;

Vertommen, Didier ;

Fortpied, Juliette ;

Duester, Gregg ;

Van Schaftingen, Emile .

BIOCHIMIE, 2007, 89 (03) :369-373

[9] ENHANCED MOTILITY IN NIH-3T3 FIBROBLASTS THAT OVEREXPRESS GELSOLIN [J].

CUNNINGHAM, CC ;

STOSSEL, TP ;

KWIATKOWSKI, DJ .

SCIENCE, 1991, 251 (4998) :1233-1236

[10]

Dosaka-Akita H, 1998, CANCER RES, V58, P322

← 1 2 3 4 5 →