Thiol-speciric and nonspecific interactions between DNA and gold nanoparticles

被引：66

作者：

Cárdenas, M

Barauskas, J

Schillén, K

Brennan, JL

Brust, M

Nylander, T

机构：

[1] Lund Univ, Lund, Sweden

[2] Univ Liverpool, Dept Chem, Ctr Nanoscale Sci, Liverpool L69 3BX, Merseyside, England

来源：

LANGMUIR | 2006年 / 22卷 / 07期

关键词：

D O I：

10.1021/la0530438

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The contribution of nonspecific interactions to the overall interactions of thiol-ssDNA and dsDNA macromolecules with gold nanoparticles was investigated. A systematic investigation utilizing dynamic light scattering and cryogenic transmission electron microscopy has been per-formed to directly measure and visualize the changes in particle size and appearance during functionalization of gold nanoparticles with thiol-ssDNA and nonthiolated dsDNA. The results show that both thiol-ssDNA and dsDNA do stabilize gold nanoparticle dispersions, but possible nonspecific interactions between the hydrophobic DNA bases and the gold surface promote interparticle interactions and cause aggregation within rather a short period of time. We also discuss the adsorption mechanisms of dsDNA and thiol-ssDNA to gold particles.

引用

收藏

页码：3294 / 3299

页数：6

相关论文

共 34 条

[1] Ionic effects on the elasticity of single DNA molecules [J].

Baumann, CG ;

Smith, SB ;

Bloomfield, VA ;

Bustamante, C .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (12) :6185-6190

[2] DNA-modified core-shell Ag/Au nanoparticles [J].

Cao, YW ;

Jin, R ;

Mirkin, CA .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2001, 123 (32) :7961-7962

[3] Interaction between DNA and charged colloids could be hydrophobically driven [J].

Cárdenas, M ;

Schillén, K ;

Pebalk, D ;

Nylander, T ;

Lindman, B .

BIOMACROMOLECULES, 2005, 6 (02) :832-837

[4] DNA compaction by cationic surfactant in solution and at polystyrene particle solution interfaces:: a dynamic light scattering study [J].

Cárdenas, M ;

Schillén, K ;

Nylander, T ;

Jansson, J ;

Lindman, B .

PHYSICAL CHEMISTRY CHEMICAL PHYSICS, 2004, 6 (07) :1603-1607

[5]

DEAN HJ, 2003, COMPUT IMMUNOL MICRO, V26, P832

[6] A fluorescence-based method for determining the surface coverage and hybridization efficiency of thiol-capped oligonucleotides bound to gold thin films and nanoparticles [J].

Demers, LM ;

Mirkin, CA ;

Mucic, RC ;

Reynolds, RA ;

Letsinger, RL ;

Elghanian, R ;

Viswanadham, G .

ANALYTICAL CHEMISTRY, 2000, 72 (22) :5535-5541

[7] CONTROLLED NUCLEATION FOR REGULATION OF PARTICLE-SIZE IN MONODISPERSE GOLD SUSPENSIONS [J].

FRENS, G .

NATURE-PHYSICAL SCIENCE, 1973, 241 (105) :20-22

[8] Characterization of DNA probes immobilized on gold surfaces [J].

Herne, TM ;

Tarlov, MJ .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1997, 119 (38) :8916-8920

[9] The interaction between PEO-PPO-PEO triblock copolymers and ionic surfactants in aqueous solution studied using light scattering and calorimetry [J].

Jansson, J ;

Schillén, K ;

Olofsson, G ;

da Silva, RC ;

Loh, W .

JOURNAL OF PHYSICAL CHEMISTRY B, 2004, 108 (01) :82-92

[10]

JOHNSEN RM, 1992, LASER LIGHT SCATTERI, P449

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