Identification of genes over-expressed in small cell lung carcinoma using suppression subtractive hybridization and cDNA microarray expression analysis

被引：57

作者：

Bangur, CS

Switzer, A

Fan, LQ

Marton, MJ

Meyer, MR

Wang, TT

机构：

[1] Corixa Corp, Tumor Antigen Discovery, Seattle, WA 98104 USA

[2] Rosetta Inpharm, Washington, WA 98034 USA

来源：

ONCOGENE | 2002年 / 21卷 / 23期

关键词：

small cell lung carcinoma (SCLC); microarray; subtractive hybridization; real-time PCR; gene expression;

D O I：

10.1038/sj.onc.1205480

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

To identify genes that are differentially over-expressed in Small Cell Lung Carcinoma (SCLC) we have used a combination of suppression subtractive hybridization and cDNA microarray to analyse the expression profiles of 2400 cDNAs clones. Genes that are over-expressed in SCLC were identified using 32 pairs of fluorescence-labeled cDNA samples representing various lung tumors and normal tissues. This comprehensive approach has resulted in the identification of 209 genes that are differentially over-expressed in SCLC. Quantitative real-time PCR was used to further validate the expression of 43 genes in SCLC tumors and various normal tissues. Discussed in this report are nine genes, which showed the most promising SCLC tumor to normal tissue differential expression profiles, including seven known and two novel genes. The large number of differentially expressed genes identified from this analysis and the characterization of these genes will provide valuable information in better understanding the biology of SCLC and help us in developing these gene products as potential targets for diagnostic as well as therapeutic usage.

引用

页码：3814 / 3825

页数：12

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