Effects of repeated silver nanoparticles exposure on the histological structure and mucins of nasal respiratory mucosa in rats

被引:76
作者
Hyun, Jin-Sook [2 ]
Lee, Byoung Seok [3 ]
Ryu, Hyeon Yeol [1 ]
Sung, Jae Hyuck [1 ]
Chung, Kyu Hyuck [2 ]
Yu, Il Je [1 ,2 ]
机构
[1] Korea Environm & Merchandise Testing Inst, Inchon 406840, South Korea
[2] Sungkyunkwan Univ, Coll Pharm, Suwon, South Korea
[3] Korea Inst Toxicol, Taejon, South Korea
关键词
Silver nanoparticle; Inhalation; Nasal respiratory mucosa; Mucin; Neutral mucin; Acid mucin;
D O I
10.1016/j.toxlet.2008.08.003
中图分类号
R99 [毒物学(毒理学)];
学科分类号
100405 ;
摘要
To investigate the effects of repeated silver nanoparticle exposure on the nasal Septum respiratory mucosa, 6-week-old SD rats were exposed to silver nanoparticles at concentrations of fresh air control, low-dose (1.73 x 10(4)/cm. 0.5 mu g/m(3)), middle-close (1.27 x 10(5)/cm(3), 3.5 mu g/m(3)) and high-dose (1.32 x 10(6) particles/cm(3), 61 mu g/m(3)) in an inhalation chamber for 6 h per day, 5 times a week for 28 days. The animals were sacrificed after the 28 days of exposure period. Histochernical staining, including periodic acid Schiff (PAS), alcian blue (AB) pH 2.5, and high iron diamine-alcian blue (HID-AB) pH 2.5, was used to evaluate changes in the mucosubstance properties of the goblet cells in the respiratory epithelium. in a histopathological study, the nasal cavity and lungs from the exposed groups exhibited no remarkable changes Compared to the control group. However, a slight increase in the neutral mucins was noted for all the silver nanoparticle-exposed groups when compared to the control group, although without statistical significance. Nonetheless, the size and number of goblet cells containing neutral mucins increased significantly in the groups exposed to silver nanoparticle at middle- and high-close (P< 0.05). While the densities of the stained mucosubstances showed no difference among the exposed groups, the amount of neutral mucins did tend to increase slightly, although acid mucins including sulfomucins and sialomucins showed no change in any of the exposed groups. Therefore, the present results did indicate that silver nanoparticles have an influence on the neutral mucins in the respiratory mucosa, yet without toxicological significance. (C) 2008 Elsevier Ireland Ltd. All rights reserved.
引用
收藏
页码:24 / 28
页数:5
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