Antibody-metalloporphyrin catalytic assembly mimics natural oxidation enzymes

被引：65

作者：

Nimri, S

Keinan, E ^{[1
]}

机构：

[1] Technion Israel Inst Technol, Dept Chem, IL-32000 Haifa, Israel

[2] Technion Israel Inst Technol, Inst Catalysis Sci & Technol, IL-32000 Haifa, Israel

[3] Scripps Res Inst, Dept Mol Biol, La Jolla, CA 92037 USA

[4] Scripps Res Inst, Skaggs Inst Chem Biol, La Jolla, CA 92037 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 1999年 / 121卷 / 39期

关键词：

D O I：

10.1021/ja990314q

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

An antibody-metalloporphynin assembly that catalyzes the enantioselective oxidation of aromatic sulfides to sulfoxides is presented. Antibody SN37.4 was elicited against a water-soluble tin(IV) porphyrin containing an axial alpha-naphthoxy ligand. The catalytic assembly comprising antibody SN37.4 and a ruthenium(II) porphyrin cofactor exhibited typical enzyme characteristics, such as predetermined oxidant and substrate selectivity, enantioselective delivery of oxygen to the substrate, and Michaelis-Menten saturation kinetics. This assembly, which promotes a complex, multistep catalytic event, represents a close model of natural heme-dependent oxidation enzymes.

引用

页码：8978 / 8982

页数：5

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