Molecular analysis of 16S-23S spacer regions of Acetobacter species

被引：4

作者：

Kretová, M ^{[1
]}

Grones, J ^{[1
]}

机构：

[1] Comenius Univ, Fac Sci, Dept Mol Biol, Bratislava 84215, Slovakia

来源：

FOLIA MICROBIOLOGICA | 2005年 / 50卷 / 04期

关键词：

D O I：

10.1007/BF02931408

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

16S-23S rDNA internal transcribed spacer regions (ITS) similarities were determined in 8 Acetobacter and 1 Glueonacetobacter strains. ITS-PCR amplification of the 16S-23S spacers showed 2 products of similar size in 7 strains; only 1 product of similar size was found in the 2 remaining strains. Analysis of the PCR products using restriction endonucleases HaeIII, HpaII and AluI revealed 3 different restriction groups of A. pasteurianus for AluI and HaeIII, and 4 restriction groups for HpaII, ITS nucleotide sequences of all studied strains exhibited a 52-98 % similarity.

引用

页码：288 / 292

页数：5

共 24 条

[1]

BIRNBOIM HC, 1979, NUCLEIC ACIDS RES, V7, P1513

[2]

BULLOCK WO, 1987, BIOTECHNIQUES, V5, P376

[3]

Cleenwerck I, 2002, INT J SYST EVOL MICR, V52, P1551, DOI [10.1099/ijs.0.02064-0, 10.1099/00207713-52-5-1551]

[4]

De Ley J, 1984, BERGEYS MANUAL SYSTE, V1, P267

[5] INTRA GENERIC AND INTERGENERIC SIMILARITIES OF THE RIBOSOMAL RIBONUCLEIC-ACID CISTRONS OF ACETOBACTER AND GLUCONOBACTER [J].

GILLIS, M ;

DELEY, J .

INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1980, 30 (01) :7-27

[6]

Holt G.J., 1994, Bergey's Manual of Determinative Bacteriology

[7] RAPID IDENTIFICATION OF BACTERIA ON THE BASIS OF POLYMERASE CHAIN REACTION-AMPLIFIED RIBOSOMAL DNA SPACER POLYMORPHISMS [J].

JENSEN, MA ;

WEBSTER, JA ;

STRAUS, N .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1993, 59 (04) :945-952

[8] Asaia siamensis sp, nov., an acetic acid bacterium in the α-Proteobacteria [J].

Katsura, K ;

Kawasaki, H ;

Potacharoen, W ;

Saono, S ;

Seki, T ;

Yamada, Y ;

Uchimura, T ;

Komagata, K .

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2001, 51 :559-563

[9]

Lisdiyanti P, 2002, INT J SYST EVOL MICR, V52, P813, DOI [10.1099/ijs.0.01982-0, 10.1099/00207713-52-3-813]

[10] Identification of Acetobacter strains isolated from Indonesian sources, and proposals of Acetobacter syzygii sp nov., Acetobacter cibinongensis sp nov., and Acetobacter orientalis sp nov. [J].

Lisdiyanti, P ;

Kawasaki, H ;

Seki, T ;

Yamada, Y ;

Uchimura, T ;

Komagata, K .

JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY, 2001, 47 (03) :119-131

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