Three-Dimensional Ingrowth of Bone Cells Within Biodegradable Cryogel Scaffolds in Bioreactors at Different Regimes

被引：73

作者：

Bolgen, Nimet ^{[1
]}

Yang, Ying ^{[2
]}

Korkusuz, Peter ^{[3
]}

Guzel, Elif ^{[3
]}

El Haj, Alicia J. ^{[2
]}

Piskin, Erhan ^{[1
]}

机构：

[1] Hacettepe Univ, Dept Chem Engn, Div Bioengn, TR-06800 Ankara, Turkey

[2] Keele Univ, Inst Sci & Technol Med, Stoke On Trent, Staffs, England

[3] Hacettepe Univ, Fac Med, Dept Histol & Embryol, TR-06800 Ankara, Turkey

来源：

TISSUE ENGINEERING PART A | 2008年 / 14卷 / 10期

关键词：

D O I：

10.1089/ten.tea.2007.0277

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

Three-dimensional cell ingrowth within biodegradable cryogel scaffolds made of cross-linked 2-hydroxyethyl methacrylate (HEMA)-lactate-dextran with interconnected macropores was studied in bioreactors at different regimes (static, perfusion, and compression-perfusion). An osteoblast-like cell line (MG63) was used in these studies. The samples taken after selected times from the bioreactors were examined by microscopy techniques (light, SEM, TEM, and laser scanning confocal). The cell culture conditions were found to have a significant impact not only on the cell morphology, such as the extent of cell attachment and ingrowth, but also on cellular activities. Dynamic conditions (perfusion and/or compression) greatly improved cell ingrowth and extracellular matrix (ECM) synthesis. Alkaline phosphatase activity results confirmed the positive effect of dynamic conditions on bone cells.

引用

页码：1743 / 1750

页数：8

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