Metabolic characterization of rumen epithelial tissue from dairy calves fed different starter diets using 1H NMR spectroscopy

被引:10
作者
Bertram, Hanne Christine [1 ]
Kristensen, Niels Bastian [2 ]
Vestergaard, Mogens [2 ]
Jensen, Soren Krogh [2 ]
Sehested, Jakob [2 ]
Nielsen, Niels Christian [3 ,4 ]
Malmendal, Anders [3 ,4 ]
机构
[1] Univ Aarhus, Fac Agr Sci, Dept Food Sci, DK-8830 Tjele, Denmark
[2] Univ Aarhus, Fac Agr Sci, Dept Anim Hlth Welfare & Nutr, DK-8830 Tjele, Denmark
[3] Univ Aarhus, Ctr Insoluble Protein Struct inSPIN, Interdisciplinaty Nanosci Ctr iNANO, DK-8000 Aarhus, Denmark
[4] Univ Aarhus, Dept Chem, DK-8000 Aarhus, Denmark
基金
新加坡国家研究基金会;
关键词
Metabonomics; NMR; Extracts; Cattle; Milk allowance; Starch; Short-chain fatty acids; REGRESSION; GLUTAMINE; NMR;
D O I
10.1016/j.livsci.2008.05.001
中图分类号
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号
0905 ;
摘要
The metabolic profile of calf rumen epithelial tissue was for the first time characterized by H-1 NMR spectroscopy of chloroform/methanol extracts. The metabolite profiles comprised a number of amino acids, creatine, taurine, short-chain fatty acids and triglycerides. The effects of two dietary interventions; i) four levels of milk allowance with a concomitant different uptake of starter concentrate, and ii) two diets with varying content of starch and fibre in the concentrate, were elucidated. Partial least square regression analysis revealed that the intensity of NMR signals assigned to leucine, isoleucine and valine (0.90 ppm), propionate (1.06 and 2.18 ppm), lactate (1.32 ppm), butyrate (1.56 ppm), acetate (1.93 ppm), glutamine/glutamate (2.18, 2.35 and 3.75 ppm), creatine (3.04 and 3.94 ppm) and glycine (3.55 ppm) decreased with increasing milk allowance, In addition, the analysis revealed that the main difference between the two diets was the content of propionate in the epithelia tissue extracts. Previous morphological analyses of the same rumen epithelia were not able to detect any significant effects of either milk allowance or dietary starch content. Accordingly, the present study demonstrated that H-1 NMR spectroscopy applied on extracts is a useful tool for metabolite profiling of epithelial tissue and for following the development of epithelia tissue in Young calves, and that the technique may be more sensitive to dietary effects than morphological Studies.,(c) 2009 Elsevier B.V. All rights reserved.
引用
收藏
页码:127 / 134
页数:8
相关论文
共 24 条
[1]   2-DIMENSIONAL SPECTROSCOPY - APPLICATION TO NUCLEAR MAGNETIC-RESONANCE [J].
AUE, WP ;
BARTHOLDI, E ;
ERNST, RR .
JOURNAL OF CHEMICAL PHYSICS, 1976, 64 (05) :2229-2246
[2]   MLEV-17-BASED TWO-DIMENSIONAL HOMONUCLEAR MAGNETIZATION TRANSFER SPECTROSCOPY [J].
BAX, A ;
DAVIS, DG .
JOURNAL OF MAGNETIC RESONANCE, 1985, 65 (02) :355-360
[3]   Effect of magnetic field strength on NMR-Based metabonomic human urine data comparative study of 250, 400, 500, and 800 MHz [J].
Bertram, Hanne Christine ;
Malmendal, Anders ;
Petersen, Bent O. ;
Madsen, Jens Chr. ;
Pedersen, Henrik ;
Nielsen, Niels Chr. ;
Hoppe, Camilla ;
Molgaard, Christian ;
Michaelsen, Kim F. ;
Duus, Jens O. .
ANALYTICAL CHEMISTRY, 2007, 79 (18) :7110-7115
[4]   A metabolomic investigation of splanchnic metabolism using 1H NMR spectroscopy of bovine blood plasma [J].
Bertram, HC ;
Kristensen, NB ;
Malmendal, A ;
Nielsen, NC ;
Bro, R ;
Andersen, HJ ;
Harmon, DL .
ANALYTICA CHIMICA ACTA, 2005, 536 (1-2) :1-6
[5]  
Blikslager AT, 1999, SURGERY, V125, P186, DOI 10.1067/msy.2099.93790
[6]   COHERENCE TRANSFER BY ISOTROPIC MIXING - APPLICATION TO PROTON CORRELATION SPECTROSCOPY [J].
BRAUNSCHWEILER, L ;
ERNST, RR .
JOURNAL OF MAGNETIC RESONANCE, 1983, 53 (03) :521-528
[7]   SUPPRESSION OF CROSS-RELAXATION EFFECTS IN TOCSY SPECTRA VIA A MODIFIED DIPSI-2 MIXING SEQUENCE [J].
CAVANAGH, J ;
RANCE, M .
JOURNAL OF MAGNETIC RESONANCE, 1992, 96 (03) :670-678
[8]  
Gaebel G., 2006, P173
[9]   Effect of milk allowance on concentrate intake, ruminal environment, and ruminal development in milk-fed holstein calves [J].
Kristensen, N. B. ;
Sehested, J. ;
Jensen, S. K. ;
Vestergaard, M. .
JOURNAL OF DAIRY SCIENCE, 2007, 90 (09) :4346-4355
[10]  
Kristensen N. B., 2006, P249