Presynaptic association of Rad51 protein with selected sites in meiotic chromatin

被引:89
作者
Plug, AW
Xu, JH
Reddy, G
Golub, EI
Ashley, T
机构
[1] YALE UNIV,SCH MED,DEPT GENET,NEW HAVEN,CT 06510
[2] AGR UNIV WAGENINGEN,DEPT GENET,6703 HA WAGENINGEN,NETHERLANDS
关键词
synaptic initiation; paranemic interactions;
D O I
10.1073/pnas.93.12.5920
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Eukaryotic homologs of Escherichia coli RecA protein have been shown to form nucleoprotein filaments with single-stranded DNA that recognize homologous sequences in duplex DNA. Several recent reports in four widely diverse species have demonstrated the association of RecA homologs with meiotic prophase chromatin, The current immunocytological study on mouse spermatocytes and oocytes shows that a eukaryotic homolog, Rad51, associates with a subset of chromatin sites as early as premeiotic S phase, hours before either the appearance of precursors of synaptonemal complexes or the initiation of synapsis, When homologous chromosomes do begin to pair, the Rad51-associated sequences are sites of initial contact between homologues and of localized DNA synthesis. Distribution of Rad51 foci on the chromatin of fully synapsed bivalents at early pachynema corresponds to an R-band pattern of mitotic chromosomes. R-bands are known to be preferred sites of both synaptic initiation and recombination. The time course of appearance of Rad51 association with chromatin, its distribution, and its interaction with other Rad51-associated sequences suggests that it plays an important role preselection of sequences and synaptic initiation.
引用
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页码:5920 / 5924
页数:5
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