T regulatory cell separation for clinical application

被引：35

作者：

Di Ianni, Mauro ^{[1
,2
]}

Del Papa, Beatrice ^{[2
]}

Zei, Tiziana ^{[2
]}

Ostini, Roberta Iacucci ^{[2
]}

Cecchini, Debora ^{[2
]}

Cantelmi, Maria Grazia ^{[2
]}

Baldoni, Stefano ^{[2
]}

Sportoletti, Paolo ^{[2
]}

Cavalli, Laura ^{[2
]}

Carotti, Alessandra ^{[2
]}

Pierini, Antonio ^{[2
]}

Falini, Brunangelo ^{[2
]}

Martelli, Massimo F. ^{[2
]}

Falzetti, Franca ^{[2
]}

机构：

[1] Univ Aquila, Dept Internal Med & Publ Hlth, Chair Hematol, I-67100 Laquila, Italy

[2] Univ Perugia, Dept Clin & Expt Med, Hematol & Clin Immunol Sect, I-06100 Perugia, Italy

来源：

TRANSFUSION AND APHERESIS SCIENCE | 2012年 / 47卷 / 02期

关键词：

VERSUS-HOST-DISEASE; EXPRESSION; INFUSION; FOXP3;

D O I：

10.1016/j.transci.2012.06.007

中图分类号：

R5 [内科学];

学科分类号：

100201 [内科学];

摘要：

We selected T regulatory cells (Tregs) from standard leukapheresis using double-negative selection (anti-CD8 and anti-CD19) followed by positive selection (anti-CD25) and 72 procedures were performed. A median of 263 x 10(6) cells (range 143-470 x 10(6)) were recovered with a mean of CD4(+)/CD25(+) cells of 94.5 +/- 2.4% (36.5 +/- 18.6% CD4(+)/CD25(+hi)). FoxP3(+) cells were equal to 79.8%+/- 22.2. CD127(+) cells were 12.5%+/- 8.2. The inhibition assay showed an inhibition rate of 67 +/- 22. Cells isolated by means of this approach can be used in allogeneic hematopoietic stem cell transplantation to reduce the incidence and severity of GvHD without bystander inhibition of general immunity. (C) 2012 Elsevier Ltd. All rights reserved.

引用

收藏

页码：213 / 216

页数：4

相关论文

共 28 条

[1]

[Anonymous], BLOOD

[2]

CD4+CD25high regulatory cells in human peripheral blood [J].

Baecher-Allan, C ;

Brown, JA ;

Freeman, GJ ;

Hafler, DA .

JOURNAL OF IMMUNOLOGY, 2001, 167 (03) :1245-1253

[3]

Infusion of ex vivo expanded T regulatory cells in adults transplanted with umbilical cord blood: safety profile and detection kinetics [J].

Brunstein, Claudio G. ;

Miller, Jeffrey S. ;

Cao, Qing ;

McKenna, David H. ;

Hippen, Keli L. ;

Curtsinger, Julie ;

DeFor, Todd ;

Levine, Bruce L. ;

June, Carl H. ;

Rubinstein, Pablo ;

McGlave, Philip B. ;

Blazar, Bruce R. ;

Wagner, John E. .

BLOOD, 2011, 117 (03) :1061-1070

[4]

CD4+CD25+ immunoregulatory T cells:: New therapeutics for graft-versus-host disease [J].

Cohen, JL ;

Trenado, A ;

Vasey, D ;

Klatzmann, D ;

Salomon, BL .

JOURNAL OF EXPERIMENTAL MEDICINE, 2002, 196 (03) :401-406

[5]

Immunomagnetic isolation of CD4+CD25+FoxP3+ natural T regulatory lymphocytes for clinical applications [J].

Di Ianni, M. ;

Del Papa, B. ;

Cecchini, D. ;

Bonifacio, E. ;

Moretti, L. ;

Zei, T. ;

Ostini, R. Iacucci ;

Falzetti, F. ;

Fontana, L. ;

Tagliapietra, G. ;

Maldini, C. ;

Martelli, M. F. ;

Tabilio, A. .

CLINICAL AND EXPERIMENTAL IMMUNOLOGY, 2009, 156 (02) :246-253

[6]

Adoptive Immunotherapy with Tregs and Tcons Ensures Low TRM and a Low Incidence of Post Transplant Leukaemia Relapse After HLA Haploidentical Transplants for Acute Leukemia [J].

Di Ianni, Mauro ;

Falzetti, Franca ;

Carotti, Alessandra ;

Terenzi, Adelmo ;

Ruggeri, Loredana ;

Antonio, Pierini ;

de Fazio, Vincenza ;

Zoi, Valentina ;

Massei, Maria Speranza ;

Reisner, Yair ;

Velardi, Andrea ;

Aversa, Franco ;

Martelli, Massimo Fabrizio .

BLOOD, 2011, 118 (21) :76-76

[7]

Mesenchymal cells recruit and regulate T regulatory cells [J].

Di Lanni, Mauro ;

Del Papa, Beatrice ;

De Loanni, Maria ;

Moretti, Lorenzo ;

Bonifacio, Elisabetta ;

Cecchini, Debora ;

Sportoletti, Paolo ;

Falzetti, Franca ;

Tabilio, Antonio .

EXPERIMENTAL HEMATOLOGY, 2008, 36 (03) :309-318

[8]

CD4+CD25+ regulatory T cells preserve graft-versus-tumor activity while inhibiting graft-versus-host disease after bone marrow transplantation [J].

Edinger, M ;

Hoffmann, P ;

Ermann, J ;

Drago, K ;

Fathman, CG ;

Strober, S ;

Negrin, RS .

NATURE MEDICINE, 2003, 9 (09) :1144-1150

[9]

Regulatory T cell lineage specification by the forkhead transcription factor FoxP3 [J].

Fontenot, JD ;

Rasmussen, JP ;

Williams, LM ;

Dooley, JL ;

Farr, AG ;

Rudensky, AY .

IMMUNITY, 2005, 22 (03) :329-341

[10]

Gavin MA, 2006, P NATL ACAD SCI USA, V103, P6659, DOI 10.1073/pnas.0509484103