DNA methylation-dependent chromatin fiber compaction in vivo and in vitro: requirement for linker histone

被引：48

作者：

Karymov, MA

Tomschik, M

Leuba, SH

Caiafa, P

Zlatanova, J

机构：

[1] Polytech Univ, Dept Chem & Chem Engn, Brooklyn, NY 11201 USA

[2] NCI, Lab Receptor Biol & Gene Express, NIH, Bethesda, MD 20892 USA

[3] Univ Roma La Sapienza, Dept Cellular Biotechnol & Haematol, I-00161 Rome, Italy

来源：

FASEB JOURNAL | 2001年 / 15卷 / 14期

关键词：

histone H1; chromatin fibers; atomic force microscopy;

D O I：

10.1096/fj.01-0345com

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Dynamic alterations in chromatin structure mediated by postsynthetic histone modifications and DNA methylation constitute a major regulatory mechanism in DNA functioning. DNA methylation has been implicated in transcriptional silencing, in part by inducing chromatin condensation. To understand the methylation-dependent chromatin structure, we performed atomic force microscope (AFM) studies of fibers isolated from cultured cells containing normal or elevated levels of m(5)C. Chromatin fibers were reconstituted on control or methylated DNA templates in the presence or absence of linker histone. Visual inspection of AFM images, combined with quantitative analysis of fiber structural parameters, suggested that DNA methylation induced fiber compaction only in the presence of linker histones. This conclusion was further substantiated by biochemical results.

引用

页码：2631 / 2641

页数：11

共 60 条

[1] Linker histone protects linker DNA on only one side of the core particle and in a sequence-dependent manner [J].