Isolation of cell type-specific apoptotic bodies by fluorescence-activated cell sorting

被引：83

作者：

Atkin-Smith, Georgia K. ^{[1
]}

Paone, Stephanie ^{[1
]}

Zanker, Damien J. ^{[1
]}

Duan, Mubing ^{[1
]}

Phan, Than K. ^{[1
]}

Chen, Weisan ^{[1
]}

Hulett, Mark D. ^{[1
]}

Poon, Ivan K. H. ^{[1
]}

机构：

[1] La Trobe Univ, La Trobe Inst Mol Sci, Dept Biochem & Genet, Melbourne, Vic 3086, Australia

来源：

SCIENTIFIC REPORTS | 2017年 / 7卷

基金：

英国医学研究理事会;

关键词：

CONTRACTION; VESICLES; BLEBS; DEATH;

D O I：

10.1038/srep39846

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

070301 [无机化学]; 070403 [天体物理学]; 070507 [自然资源与国土空间规划学]; 090105 [作物生产系统与生态工程];

摘要：

Apoptotic bodies (ApoBDs) are membrane-bound extracellular vesicles that can mediate intercellular communication in physiological and pathological settings. By combining recently developed analytical strategies with fluorescence-activated cell sorting (FACS), we have developed a method that enables the isolation of ApoBDs from cultured cells to 99% purity. In addition, this approach also enables the identification and isolation of cell type-specific ApoBDs from tissue, bodily fluid and blood-derived samples.

引用

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