Molecular identification of adenoviruses in clinical samples by analyzing a partial hexon genomic region

被引：65

作者：

Casas, I

Avellon, A

Mosquera, M

Jabado, O

Echevarria, JE

Campos, RH

Rewers, M

Perez-Breña, P

Lipkin, WI

Palacios, G

机构：

[1] Columbia Univ, Mailman Sch Publ Hlth, Jerome L & Dawn Greene Infect Dis Lab, New York, NY 10032 USA

[2] ISCII, Ctr Nacl Microbiol, Serv Virol, Madrid, Spain

[3] ISCII, Ctr Nacl Microbiol, Serv Microbiol Diagnost, Madrid, Spain

[4] Univ Buenos Aires, Fac Farm & Bioquim, Catedra Virol, Buenos Aires, DF, Argentina

[5] Univ Colorado, Ctr Hlth Sci, Barbara Davis Ctr Diabet Res, Denver, CO 80202 USA

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2005年 / 43卷 / 12期

关键词：

D O I：

10.1128/JCM.43.12.6176-6182.2005

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Here we present a system for adenovirus detection and genotyping based on PCR amplification and phylogenetic analysis of a conserved hexon gene fragment. The system was validated using 157 sequences (86 previously typed and 71 clinical samples) and correctly identified species and serotype in 100% and 84% of sequences, respectively. Known associations between specific serotypes and clinical syndromes are verified. Possible new associations are described to allow further independent testing.

引用

页码：6176 / 6182

页数：7

共 24 条

[1] SIMPLE PROCEDURE FOR DISCRIMINATION AND TYPING OF ENTERIC ADENOVIRUSES AFTER DETECTION BY POLYMERASE CHAIN-REACTION [J].